Electrochemical and spectroelectrochemical characterization of bacteria and bacterial systems.
Electrochemical and spectroelectrochemical characterization of bacteria and bacterial systems.
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DOI：
10.1039/d1an01954f
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发表时间：
2021-12-20
期刊：
影响因子：
--
通讯作者：
Bohn PW
中科院分区：
文献类型：
--
作者：
Sundaresan V;Do H;Shrout JD;Bohn PW
微生物，例如细菌，在某种程度上可以被描述为小型的、自我维持的化工厂。根据物种、菌株甚至环境，细菌对人类生命可能是有用的、中性的或致病的，因此，我们能够在分子水平上以化学特异性和时空分辨率来表征它们，以了解它们的行为，这一点变得越来越重要。细菌代谢涉及大量的内部和外部电子转移过程，因此采用电化学技术来研究这些细菌代谢物是合乎逻辑的。在这篇小综述中，我们重点关注专门用于化学表征细菌及其行为的电化学和光谱电化学方法。首先，我们讨论微生物电子转移的最新机制见解和当前的理解，包括直接电子转移和介导电子转移。其次，我们总结了分泌因子（包括代谢物和信号分子）时空表征方法的进展，这些方法可用于辨别自然或外部因素如何改变细菌细胞的代谢状态并改变其个体或集体行为。最后，我们讨论了单细胞表征的原位方法，该方法可以揭示细胞行为的异质性如何反映在细菌集合的行为和特性中，例如细菌的行为和特性。细菌群落。细菌（光谱）电化学表征的最新进展在整体和单一实体水平上产生了重要的新见解，这进一步加深了我们对细菌行为的理解。这些见解反过来有望使从生物传感器到在基于细菌的生物能源生成和存储中使用细菌等应用受益。本综述重点关注已开发并专门用于化学表征细菌及其行为的电化学和光谱电化学方法。
Microbes, such as bacteria, can be described, at one level, as small, self-sustaining chemical factories. Based on the species, strain, and even the environment, bacteria can be useful, neutral or pathogenic to human life, so it is increasingly important that we be able to characterize them at the molecular level with chemical specificity and spatial and temporal resolution in order to understand their behavior. Bacterial metabolism involves a large number of internal and external electron transfer processes, so it is logical that electrochemical techniques have been employed to investigate these bacterial metabolites. In this mini-review, we focus on electrochemical and spectroelectrochemical methods that have been developed and used specifically to chemically characterize bacteria and their behavior. First, we discuss the latest mechanistic insights and current understanding of microbial electron transfer, including both direct and mediated electron transfer. Second, we summarize progress on approaches to spatiotemporal characterization of secreted factors, including both metabolites and signaling molecules, which can be used to discern how natural or external factors can alter metabolic states of bacterial cells and change either their individual or collective behavior. Finally, we address in situ methods of single-cell characterization, which can uncover how heterogeneity in cell behavior is reflected in the behavior and properties of collections of bacteria, e.g. bacterial communities. Recent advances in (spectro)electrochemical characterization of bacteria have yielded important new insights both at the ensemble and the single-entity levels, which are furthering our understanding of bacterial behavior. These insights, in turn, promise to benefit applications ranging from biosensors to the use of bacteria in bacteria-based bioenergy generation and storage. This review focuses on electrochemical and spectroelectrochemical methods that have been developed and used specifically to chemically characterize bacteria and their behavior.
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影响因子：
3.4
作者：
Brand, Izabella;Khairalla, Bishoy
通讯作者：
Khairalla, Bishoy
影响因子：
15
作者：
Baker LA
通讯作者：
Baker LA
影响因子：
6.7
作者：
Duddy OP;Bassler BL
通讯作者：
Bassler BL
影响因子：
4.6
作者：
Buzid A;Shang F;Reen FJ;Muimhneacháin EÓ;Clarke SL;Zhou L;Luong JH;O'Gara F;McGlacken GP;Glennon JD
通讯作者：
Glennon JD
影响因子：
8.4
作者：
Bond, Daniel R.;Strycharz-Glaven, Sarah M.;Torres, Cesar I.
通讯作者：
Torres, Cesar I.