Biodegradation of Enteromorpha polysaccharides by intestinal micro-community from Siganus oramin
Biodegradation of Enteromorpha polysaccharides by intestinal micro-community from Siganus oramin
复制标题
浒苔肠道微群落生物降解浒苔多糖
DOI：
10.1007/s11802-016-3002-0
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发表时间：
2016-11
影响因子：
1.6
通讯作者：
Hu, Zhong
中科院分区：
文献类型：
--
作者：
Xu, Yan;Li, Jin;Li, Yuanyou;Hu, Zhong
肠道微生物群落被认为比单一菌株具有更大的降解多糖的潜在功能，但肠道微生物群落参与浒苔多糖（Enteromorpha polysaccharides，EP）降解的研究报道较少。为了获得EP降解菌群，取橙色蓝子鱼肠道，分离出富集0.3% EP作为唯一碳源的微生物群。经过7代的传代培养，获得了一个稳定的具有EP降解能力的微群落，命名为H1。结果表明，H1在24 h内能够降解75%的EP，32 h内EP裂解酶的活性达到500 U mL−1。通过变性梯度凝胶电泳（DGGE）和16 S rRNA基因克隆文库分析，从10株与海单胞菌（Marinomonaspontica）、微杆菌属（Microbacterium sp.）Chironomi白杆菌，Cyclobacterium sp.，检测出嗜银螨、假交替单胞菌和弧菌。进一步通过DGGE条带序列分析和克隆文库分析，确定该微群落的优势菌为假交替单胞菌（Pseudoalteromonas sp.）和弧菌（Vibrio sp.），在微群落H1中，它们分别占38%和46%，应该与其它降解菌一起在EP降解中发挥重要作用。
Micro-communities are supposed to have more potential functions of biodegradation of polysaccharides than single strain; however, the intestinal micro-communities involved in the biodegradation of Enteromorpha polysaccharides (EP) were seldom reported. In order to obtain the EP-degrading micro-community, the intestines of Siganus oramin was obtained to isolate the micro-communities, which were enriched by 0.3% of EP as the sole carbon source. A stable micro-community with EP degradative capability was achieved after seven generations of subculture, named H1. Results showed that H1 was able to degrade 75% of EP within 24 hours, and the activity of EP lyases reached 500 U mL−1 in 32 hours. With denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis, ten bacteria closely related to Marinomonas pontica, Microbacterium sp., Leucobacter chironomi, Cyclobacterium sp., Algoriphagus winogradskyi, Pseudoalteromonas sp. and Vibrio sp. were determined. Furthermore, compared with the DGGE bands sequence and the clone library analysis, the dominant bacteria of the EP-biodegrading micro- community were Pseudoalteromonas sp. and Vibrio sp., with the respective proportion of 38% and 46%, and they should play an important role in EP degradation together with other degrading bacteria in the micro-community H1.
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DOI：
10.1016/j.chemolab.2005.04.005
发表时间：
2005-10-28
影响因子：
3.9
作者：
Mecozzi, M
通讯作者：
Mecozzi, M
影响因子：
4.4
作者：
Yasushi Sugano;T. Matsumoto;Hisashi Kodama;M. Noma
通讯作者：
Yasushi Sugano;T. Matsumoto;Hisashi Kodama;M. Noma
DOI：
10.1007/978-3-642-56968-5_4
发表时间：
2000
期刊：
Comput. Oper. Res.
影响因子：
--
作者：
S. Surzycki
通讯作者：
S. Surzycki
影响因子：
3.3
作者：
Qianqian Wu;M. Zhang;Ke Wu;B. Liu;Jingmin Cai;R. Pan
通讯作者：
Qianqian Wu;M. Zhang;Ke Wu;B. Liu;Jingmin Cai;R. Pan
影响因子：
2.8
作者：
Duan, Gaofei;Han, Feng;Yu, Wengong
通讯作者：
Yu, Wengong