The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling.
The excretory-secretory products of Echinococcus granulosus protoscoleces stimulated IL-10 production in B cells via TLR-2 signaling.
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细粒棘球蚴原头节的排泄分泌产物通过 TLR-2 信号刺激 B 细胞产生 IL-10
DOI：
10.1186/s12865-018-0267-7
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发表时间：
2018-10-24
期刊：
影响因子：
3
通讯作者：
Zheng KY
中科院分区：
文献类型：
--
作者：
Pan W;Xu HW;Hao WT;Sun FF;Qin YF;Hao SS;Liu H;Cao JP;Shen YJ;Zheng KY
背景：众所周知，细粒棘球绦虫原头节 (EgPSC-ESP) 释放的排泄分泌产物可调节 T 细胞反应。然而，它们对 B 细胞亚群分化的直接影响在很大程度上仍然难以捉摸。本研究研究了EgPSC-ESPs对产生IL-10的B细胞（B10）分化的影响，并探讨了Toll样受体2（TLR-2）信号传导在此过程中的可能作用。结果：与磷酸盐缓冲盐水（PBS）相比，暴露于排泄分泌产物（ESPs）的B细胞产生更高比例的B10细胞，IL-10 mRNA表达更高，IL-10含量更高的产生，呈剂量依赖性。 ESPs刺激的B细胞中TLR-2的mRNA和蛋白表达显着高于PBS中，这与从EgPSC感染的小鼠中分离的B细胞的结果一致。此外，与正常 B 细胞相比，TLR-2-/-B 细胞响应 ESP 刺激，表达较低水平的 IL-10 mRNA，并产生不可检测的 IL-10。此外，在 ESP 处理的 B 细胞中，十号染色体上缺失的磷酸酶和张力蛋白同源物/AKT/磷脂酰肌醇-3 激酶 (PTEN/AKT/PI3K) 通路被激活，这也依赖于 TLR-2 信号传导。 TLR-2 的激动剂 Pam3CSK4 可以模拟 ESP 对 PTEN、AKT 和 PI3K 表达的影响。结论：总体而言，本研究表明 EgPSC-ESP 介导的 B10 诱导需要 TLR-2 信号传导，而 EgPSC-ESP 可能是对抗寄生虫感染的免疫调节靶点。
Background:Excretory-secretory products released by Echinococcus granulosus protoscoleces (EgPSC-ESPs) are well-known to regulate T cell responses. However, their direct influence on the differentiation of B cell subsets remains largely elusive. This study investigated the effects of EgPSC-ESPs on the differentiation of IL-10-producing B cells (B10), and explored the possible role of Toll-like receptor 2 (TLR-2) signaling in this process.Results:In comparison to phosphate buffered saline (PBS), B cells exposed to the excretory-secretory products (ESPs) generated higher percentages of B10 cells, with higher expression of IL-10 mRNA, and larger amount of IL-10 production, which were in a dose dependent way. The mRNA and protein expression of TLR-2 in the ESPs-stimulated B cells were significantly higher than those in PBS, which was consistent to the results in B cells isolated from EgPSC infected mice. Moreover, TLR-2-/-B cells in response to ESPs stimulation expressed lower levels of IL-10 mRNA and produced undetectable IL-10 in comparison to those in normal B cells. In addition, Phosphatase and tensin homolog deleted on chromosome ten/AKT/Phosphatidylinositol-3 kinase (PTEN/AKT/PI3K) pathway was activated in ESPs-treated B cells, which was also dependent on TLR-2 signaling. Pam3CSK4, the agonist of TLR-2, could mock the effects of ESPs on the expression of PTEN, AKT and PI3K.Conclusion:Overall, this study revealed that TLR-2 signaling was required for B10 induction mediated by EgPSC-ESPs, which might be an immunomodulatory target against the parasite infection.
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影响因子：
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作者：
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