The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells.
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细粒棘球蚴原头节的排泄分泌产物直接调节B10、B17和Th17细胞的分化
DOI：
10.1186/s13071-017-2263-9
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发表时间：
2017-07-21
影响因子：
3.2
通讯作者：
Zheng KY
中科院分区：
文献类型：
--
作者：
Pan W;Hao WT;Shen YJ;Li XY;Wang YJ;Sun FF;Yin JH;Zhang J;Tang RX;Cao JP;Zheng KY
蠕虫分泌的排泄-分泌产物（ESPs）调节宿主的T细胞反应。然而，它们对幼稚T细胞，特别是B细胞分化的直接影响在很大程度上仍然未知。本研究探讨细粒棘球蚴原核表达质粒（EgPSC-ESPs）对分泌IL-10的B细胞（B10）、分泌IL-17 A的B细胞（B17）和Th 17细胞分化的影响。使用注射EgPSC的BALB/c小鼠来评估B10、B17和Th 17细胞的体内分布。体外纯化的CD 19 + B和幼稚CD 4 + T细胞在天然的、热灭活的或高碘酸盐处理的EgPSC-ESP存在下培养，并比较这些细胞亚群的分化。与对照组相比，感染小鼠的B10、B17和Th 17细胞频率更高，血清中IL-10和IL-17 A水平也更高。有趣的是，B17细胞首先被鉴定为表达CD 19 + CD 1d高。在体外，与PBS组相比，用天然ESP培养的B细胞显示出更高百分比的B10细胞，但更低百分比的B17和Th 17细胞。此外，IL-10和IL-17 A mRNA的相对表达与改变的频率一致。然而，ESP进行热灭活或高碘酸盐处理表现出相反的效果，这些细胞亚群的诱导。我们的研究结果表明，由EgPSC释放的ESP可以直接调节B10，B17和Th 17细胞的分化，这似乎是热不稳定和碳水化合物依赖性的。本文的在线版本（doi：10.1186/s13071-017-2263-9）包含补充材料，可供授权用户使用。
Excretory-secretory products (ESPs) released by helminths are well-known to regulate T cell responses in the host. However, their direct influence in the differentiation of naïve T cells, and especially B cells, remains largely unknown. This study investigated the effects of Echinococcus granulosus protoscoleces ESPs (EgPSC-ESPs) on the differentiation of IL-10-producing B cells (B10), IL-17A-producing B cells (B17) and Th17 cells. BALB/c mice injected with EgPSC were used to evaluate the in vivo profiles of B10, B17 and Th17 cells. In vitro purified CD19+ B and naïve CD4+ T cells were cultured in the presence of native, heat-inactivated or periodate-treated EgPSC-ESPs, and the differentiation of these cell subsets were compared. In contrast to the control group, infected mice showed higher frequencies of B10, B17 and Th17 cells, and higher levels of IL-10 and IL-17A in the sera. Interestingly, B17 cells were first identified to express CD19+CD1dhigh. In vitro, B cells cultured with native ESPs exhibited a higher percentage of B10 cells but lower percentage of B17 and Th17 cells compared to the PBS group. Moreover, the relative expression of IL-10 and IL-17A mRNA were consistent with the altered frequencies. However, ESPs subjected to heat-inactivation or periodate treatment exhibited an inverse effect on the induction of these cell subsets. Our findings indicate that ESPs released by EgPSC can directly regulate the differentiation of B10, B17 and Th17 cells, which appear to be heat-labile and carbohydrate-dependent. The online version of this article (doi:10.1186/s13071-017-2263-9) contains supplementary material, which is available to authorized users.
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影响因子：
30.5
作者：
Bermejo, Daniela A.;Jackson, Shaun W.;Gorosito-Serran, Melisa;Acosta-Rodriguez, Eva V.;Amezcua-Vesely, Maria C.;Sather, Blythe D.;Singh, Akhilesh K.;Khim, Socheath;Mucci, Juan;Liggitt, Denny;Campetella, Oscar;Oukka, Mohamed;Gruppi, Adriana;Rawlings, David J.
通讯作者：
Rawlings, David J.
影响因子：
3.8
作者：
Nono JK;Pletinckx K;Lutz MB;Brehm K
通讯作者：
Brehm K
影响因子：
4.4
作者：
Mangan, NE;Fallon, RE;Fallon, PG
通讯作者：
Fallon, PG
影响因子：
4.4
作者：
Lampropoulou, Vicky;Hoehlig, Kai;Fillatreau, Simon
通讯作者：
Fillatreau, Simon
影响因子：
3.8
作者：
Hotez PJ;Kamath A
通讯作者：
Kamath A