ERM/Rho protein expression in ductal breast cancer: a 15 year follow-up.
ERM/Rho protein expression in ductal breast cancer: a 15 year follow-up.
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DOI：
10.1007/s13402-013-0125-9
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发表时间：
2013-06
影响因子：
6.6
通讯作者：
Matkowski, Rafal
中科院分区：
文献类型：
--
作者：
Halon, Agnieszka;Donizy, Piotr;Surowiak, Pawel;Matkowski, Rafal
本研究的目的是检测乳腺癌（BC）患者中ERM （ezrin, moesin）和Rho （RhoA， RhoB和Cdc42）蛋白的表达，并研究这些蛋白的亚细胞定位与临床病理特征和患者生存之间的关系。通过免疫组织化学分析了85例II期导管性BC患者中ERM/Rho蛋白的表达和特异性亚细胞分布，随访15年。所有分析蛋白的免疫反应性增强与淋巴结转移的存在相关（ezrin, P = 0.047, moesin, P = 0.038, RhoA, P = 0.024, RhoB, P = 0.004和Cdc42， P = 0.047）。发现ezrin的核定位与淋巴结转移（P = 0.004）和组织学去分化（P = 0.015）相关。相反，我们发现RhoA和Cdc42的核地形以及RhoB的核周定位与淋巴结转移的缺乏密切相关（P = 0.008, P = 0.048, P = 0.001），而RhoA在BC肿瘤间质室的反应性降低与淋巴结转移的存在相关（P = 0.011）。BC细胞中ERM/Rho蛋白表达与雌激素受体（ER）、孕激素受体（PgR）或HER-2反应性无相关性。此外，ERM/Rho蛋白表达并不能预测患者的生存，但发现肿瘤间质室中RhoB过表达与较好的预后相关（P = 0.0106）。ERM/Rho免疫谱及其特异性亚细胞定位的评估可能有助于预测导管性BC患者的淋巴结转移。
The aim of this study was to examine the expression of ERM (ezrin, moesin) and Rho (RhoA, RhoB and Cdc42) proteins in breast cancer (BC) patients and to investigate the relationship between the sub-cellular localisation of these proteins and clinicopathological characteristics and patient survival. The expression and specific sub-cellular distribution of the ERM/Rho proteins was analysed by immunohistochemistry in a homogeneous group of 85 stage II ductal BC patients with a follow-up of 15 years. Enhanced immunoreactivity of all analysed proteins was found to be associated with the presence of lymph node metastases (ezrin, P = 0.047, moesin, P = 0.038, RhoA, P = 0.024, RhoB, P = 0.004 and Cdc42, P = 0.047). Nuclear localisation of ezrin was found to correlate with the presence of lymph nodes metastases (P = 0.004) and with histological de-differentiation (P = 0.015). In contrast, we found that the nuclear topography of RhoA and Cdc42, and the perinuclear localisation of RhoB, were strongly associated with a lack of nodal metastases (P = 0.008, P = 0.048, P = 0.001, respectively), whereas a decreased reactivity of RhoA in the stromal compartment of BC tumours was associated with the presence of lymph node metastases (P = 0.011). No relationship was observed between ERM/Rho protein expression and oestrogen receptor (ER), progesterone receptor (PgR) or HER-2 reactivity in the BC cells. Also, ERM/Rho protein expression did not predict patient survival, but RhoB over-expression in the stromal compartment of the tumours was found to be associated with a better prognosis (P = 0.0106). The ERM/Rho immunoprofile and the assessment of its specific sub-cellular localisation may be instrumental for the prediction of lymph node metastases in ductal BC patients.
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DOI：
10.1016/j.bbrc.2004.01.108
发表时间：
2004-03-12
影响因子：
3.1
作者：
Pan, YL;Bi, F;Fan, DM
通讯作者：
Fan, DM
DOI：
10.1007/s12253-010-9340-0
发表时间：
2011-09
期刊：
Pathology oncology research : POR
影响因子：
--
作者：
Halon A;Materna V;Drag-Zalesinska M;Nowak-Markwitz E;Gansukh T;Donizy P;Spaczynski M;Zabel M;Dietel M;Lage H;Surowiak P
通讯作者：
Surowiak P
影响因子：
11.5
作者：
Kamai, T;Yamanishi, T;Yoshida, KI
通讯作者：
Yoshida, KI
影响因子：
3.3
作者：
Elzagheid, Adam;Korkefla, Eija;Carpen, Otti
通讯作者：
Carpen, Otti
ezrin的形态学作用需要磷酸化引起的从质膜上的低聚物向单体的过渡。
DOI：
10.1083/jcb.150.1.193
发表时间：
2000-07-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gautreau A;Louvard D;Arpin M
通讯作者：
Arpin M