Fibroblast growth factor signaling in myofibroblasts differs from lipofibroblasts during alveolar septation in mice.
Fibroblast growth factor signaling in myofibroblasts differs from lipofibroblasts during alveolar septation in mice.
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小鼠肺泡间隔过程中，肌成纤维细胞中的成纤维细胞生长因子信号传导不同于脂肪成纤维细胞。
DOI：
10.1152/ajplung.00013.2015
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
McCoy,DiannM
中科院分区：
文献类型：
--
作者：
McGowan,StephenE;McCoy,DiannM
肺泡成纤维细胞以时间和空间调节的模式产生细胞外基质，以产生耐用而柔韧的气体交换表面。增殖确保了足够的细胞补充，但它们必须分化成功能不同的亚型：收缩性肌成纤维细胞（MF），其产生弹性蛋白并调节肺泡导管处的气流，以及在小鼠和大鼠中，脂肪成纤维细胞（LF），其储存中性脂质。PDGF-A是必需的，但与相邻上皮细胞或成纤维细胞内产生的其他分化因子一起起作用。我们假设，FGF受体（FGFR）的表达和功能不同的MF和LF，并有助于他们的分歧分化。而在MF中大约一半的FGFR 3是细胞外的，FGFR 2和FGFR 4主要是细胞内的。细胞内FGFR 3定位于多泡体，并且其丰度可以通过Sprouty和与热休克蛋白-90的相互作用来修改。FGF 18 mRNA在MF中更丰富，而FGF 10 mRNA在LF中占主导地位，LF还表达FGFR 1 IIIb，FGF 10的受体。FGF 18减少成纤维细胞增殖，并对培养的成纤维细胞具有趋化性。虽然PDGF受体-α（PDGFR-α）主要通过磷酸肌醇3-激酶和Akt信号传导，但作为FGFR主要信号传导途径的p42/p44 MAP激酶（Erk 1/2）影响细胞表面PDGFR-α的丰度。在MF和LF中观察到不同的FGFR和配体谱与其分化差异一致，尽管两个亚群均表达PDGFR-α。这些研究还强调了FGFR 3和PDGFR-α的特定细胞位置的重要性，这可能会改变它们在肺泡发育或修复过程中的作用。
Pulmonary alveolar fibroblasts produce extracellular matrix in a temporally and spatially regulated pattern to yield a durable yet pliable gas-exchange surface. Proliferation ensures a sufficient complement of cells, but they must differentiate into functionally distinct subtypes: contractile myofibroblasts (MF), which generate elastin and regulate air-flow at the alveolar ducts, and, in mice and rats, lipofibroblasts (LF), which store neutral lipids. PDGF-A is required but acts in conjunction with other differentiation factors arising from adjacent epithelia or within fibroblasts. We hypothesized that FGF receptor (FGFR) expression and function vary for MF and LF and contributes to their divergent differentiation. Whereas approximately half of the FGFR3 was extracellular in MF, FGFR2 and FGFR4 were primarily intracellular. Intracellular FGFR3 localized to the multivesicular body, and its abundance may be modified by Sprouty and interaction with heat shock protein-90. FGF18 mRNA is more abundant in MF, whereas FGF10 mRNA predominated in LF, which also express FGFR1 IIIb, a receptor for FGF10. FGF18 diminished fibroblast proliferation and was chemotactic for cultured fibroblasts. Although PDGF receptor-α (PDGFR-α) primarily signals through phosphoinositide 3-kinase and Akt, p42/p44 MAP kinase (Erk1/2), a major signaling pathway for FGFRs, influenced the abundance of cell-surface PDGFR-α. Observing different FGFR and ligand profiles in MF and LF is consistent with their divergent differentiation although both subpopulations express PDGFR-α. These studies also emphasize the importance of particular cellular locations of FGFR3 and PDGFR-α, which may modify their effects during alveolar development or repair.
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DOI：
10.1152/ajplung.00011.2013
发表时间：
2013-08-01
影响因子：
4.9
作者：
McGowan, Stephen E.;McCoy, Diann M.
通讯作者：
McCoy, Diann M.
影响因子：
3.9
作者：
Foldynova-Trantirkova, Silvie;Wilcox, William R.;Krejci, Pavel
通讯作者：
Krejci, Pavel
影响因子：
7.2
作者：
Boekel, Christian;Brand, Michael
通讯作者：
Brand, Michael
DOI：
10.1016/j.bbamcr.2012.10.010
发表时间：
2013-01
影响因子：
5.1
作者：
Mohapatra, Bhopal;Ahmad, Gulzar;Nadeau, Scott;Zutshi, Neha;An, Wei;Scheffe, Sarah;Dong, Lin;Feng, Dan;Goetz, Benjamin;Arya, Priyanka;Bailey, Tameka A.;Palermo, Nicholas;Borgstahl, Gloria E. O.;Natarajan, Amarnath;Raja, Srikumar M.;Naramura, Mayumi;Band, Vimla;Band, Hamid
通讯作者：
Band, Hamid
DOI：
10.1152/ajplung.00008.2009
发表时间：
2009-08-01
影响因子：
4.9
作者：
Perl, Anne-Karina T.;Gale, Emily
通讯作者：
Gale, Emily