Inhibition of proliferation and expression of T-antigen in SV40 large T-antigen gene-induced immortalized cells following transplantations.
Inhibition of proliferation and expression of T-antigen in SV40 large T-antigen gene-induced immortalized cells following transplantations.
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移植后 SV40 大 T 抗原基因诱导的永生化细胞增殖和 T 抗原表达的抑制。
DOI：
10.1016/s0304-3835(96)04595-8
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发表时间：
1997
期刊：
影响因子：
9.7
通讯作者：
Prasad,KN
中科院分区：
文献类型：
--
作者：
LaRosa,FG;Adams,FS;Krause,GE;Meyers,AD;Edwards-Prasad,J;Kumar,R;Freed,CR;Prasad,KN
用猴病毒40 （SV40）大t抗原基因（LTa）植入大鼠多巴胺生成神经细胞（1RB3AN27）和大鼠腮腺腺泡细胞（2RSG）进行永生化。这两种细胞在体外分裂并产生核LTa。为了研究细胞增殖与体内LTa表达的关系，我们将永生化的多巴胺生成神经细胞和腮腺细胞分别移植到成年sd大鼠纹状体和腮腺。移植细胞在移植后第1天出现核LTa，但在第7天和第30天没有。移植后30天未见肿瘤，H、E染色未见细胞分裂。当含有移植物的纹状体区培养时，这些细胞在镀后4天不表达LTa；然而，在3周后，当大多数宿主细胞被清除时，培养的移植物细胞表达了LTa。培养3个月后，只存在LTa细胞。这些细胞在移植前具有与1rb3an27细胞相同的形态和分裂倍增时间。结果表明体内存在LTa抑制因子，并支持LTa的表达与永生化细胞增殖直接相关的假设。
Rat dopamine-producing nerve cells (1RB3AN27) and rat parotid acinar cells (2RSG) were immortalized by insertion of simian virus 40 (SV40) large T-antigen gene (LTa). Both of these cells divided and produced nuclear LTa in vitro. In order to assess the relationship between cell proliferation and expression of LTa in vivo, immortalized dopamine-producing nerve cells and parotid cells were grafted into the striatum and parotid gland of adult Sprague-Dawley rats, respectively. Grafted cells exhibited nuclear LTa at 1 day but not at 7 and 30 days after transplantation. At 30 days after transplantation, no tumor was found, and there was no evidence of cell division as determined by H and E staining. When the striatal areas containing the grafts were cultured, these cells did not express LTa at 4 days after plating; however, after 3 weeks, when most host cells were eliminated, the cultured grafted cells expressed LTa. After 3 months of culturing, only cells exhibiting LTa were present. These cells had the same morphology and divided with the same doubling time as 1RB3AN27cells before grafting. Results suggest the presence of a LTa-inhibiting factor in vivo, and support the hypothesis that the expression of LTa is directly linked with proliferation of immortalized cells.
猿猴病毒 40 转化基因的片段有利于大鼠胚胎细胞的转化。
DOI：
10.1073/pnas.79.17.5189
发表时间：
1982
影响因子：
11.1
作者：
Colby,WW;Shenk,T
通讯作者：
Shenk,T
SV40 大 T 抗原基因的片段转化
影响因子：
64.8
作者：
C. Clayton;D. Murphy;M. Lovett;P. Rigby
通讯作者：
P. Rigby
DiI 作为细胞移植到实体器官的标记物。
影响因子：
2.7
作者：
Ledley,FD;Soriano,HE;O'MalleyJr,BW;Lewis,D;Darlington,GJ;Finegold,M
通讯作者：
Finegold,M