A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.
A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.
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DOI：
10.1371/journal.pone.0111789
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
McInerny CJ
中科院分区：
文献类型：
--
作者：
Bhutta MS;Roy B;Gould GW;McInerny CJ
胞质分裂和细胞分离是细胞周期中的关键事件。我们发现运输所需的内体分选复合体(ESCRT)基因是裂殖酵母细胞分离所必需的。我们确定了ESCRT蛋白与Polo和极光蛋白激酶以及CDC14磷酸酶之间的遗传相互作用，这些相互作用表现为生长受损和加剧隔膜缺陷，表明编码的蛋白质共同发挥作用来控制这些过程。此外，我们在plo1、ark1和clp1的突变体中观察到了缺陷的内体分选，这与ESCRT突变体在控制ESCRT膜运输功能中的作用是一致的。多项研究表明，POLO和ESCRT组分之间存在功能相互作用：首先，已报道Plo1p与Sst4p、Vps28p、Vps25p、Vps20p和Vps32p之间存在体内双杂交相互作用；其次，人Plk1共沉淀人的Vps20p、Vps32p、Vps24p和Vps2p同源物；以及第三，Polo激酶体外对发芽酵母Vps32p和Vps20p进行磷酸化。双杂交分析也证实了Ark1p与Vps20p和Vps32p以及Clp1p和Vps28p之间的相互作用。这些实验表明ESCRT蛋白、plo1、ark1和CLP1之间存在相互作用的网络，它们协调分裂酵母中的膜运输和细胞分离。
Cytokinesis and cell separation are critical events in the cell cycle. We show that Endosomal Sorting Complex Required for Transport (ESCRT) genes are required for cell separation in Schizosaccharomyces pombe. We identify genetic interactions between ESCRT proteins and polo and aurora kinases and Cdc14 phosphatase that manifest as impaired growth and exacerbated defects in septation, suggesting that the encoded proteins function together to control these processes. Furthermore, we observed defective endosomal sorting in mutants of plo1, ark1 and clp1, as has been reported for ESCRT mutants, consistent with a role for these kinases in the control of ESCRT function in membrane traffic. Multiple observations indicate functional interplay between polo and ESCRT components: firstly, two-hybrid in vivo interactions are reported between Plo1p and Sst4p, Vps28p, Vps25p, Vps20p and Vps32p; secondly, co-immunoprecipitation of human homologues of Vps20p, Vps32p, Vps24p and Vps2p by human Plk1; and thirdly, in vitro phosphorylation of budding yeast Vps32p and Vps20p by polo kinase. Two-hybrid analyses also identified interactions between Ark1p and Vps20p and Vps32p, and Clp1p and Vps28p. These experiments indicate a network of interactions between ESCRT proteins, plo1, ark1 and clp1 that coordinate membrane trafficking and cell separation in fission yeast.
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影响因子：
56.9
作者：
Guizetti, Julien;Schermelleh, Lothar;Gerlich, Daniel W.
通讯作者：
Gerlich, Daniel W.
DOI：
10.1038/nrm2834
发表时间：
2010-02
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
7.3
作者：
Koch, Andre;Krug, Karsten;Hauf, Silke
通讯作者：
Hauf, Silke
影响因子：
3.3
作者：
Hu CK;Ozlü N;Coughlin M;Steen JJ;Mitchison TJ
通讯作者：
Mitchison TJ
ESCRT-III复合物的膜分裂。
影响因子：
64.8
作者：
Wollert, Thomas;Wunder, Christian;Lippincott-Schwartz, Jennifer;Hurley, James H.
通讯作者：
Hurley, James H.