Topography-assisted electromagnetic platform for blood-to-PCR in a droplet.
Topography-assisted electromagnetic platform for blood-to-PCR in a droplet.
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DOI：
10.1016/j.bios.2013.06.011
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发表时间：
2013-12-15
影响因子：
12.6
通讯作者：
Wang, Tza-Huei
中科院分区：
文献类型：
--
作者：
Chiou, Chi-Han;Shin, Dong Jin;Zhang, Yi;Wang, Tza-Huei
本文提出了一种用于自动化样品制备和核酸检测的电磁驱动平台。所提出的平台将使用二氧化硅涂层磁性颗粒的核酸提取与实时聚合酶链式反应（PCR）集成在单个试剂盒上。通过使用一系列平面线圈电磁体在地形特征的辅助下操纵液滴中的磁性颗粒，基因组材料的提取实现自动化，从而能够对各种缓冲液和试剂进行高效的流体处理。通过从全血中提取核酸，然后对 KRAS 癌基因进行实时 PCR 检测，证明了该平台的功能。从全血到 PCR 就绪液滴的自动化样品处理在 15 分钟内完成。我们采用模块化方法，将磁操纵和光学检测模块与设备本身解耦，从而实现与简单外部仪器协同运行的低复杂性盒。
This paper presents an electromagnetically actuated platform for automated sample preparation and detection of nucleic acids. The proposed platform integrates nucleic acid extraction using silica-coated magnetic particles with real-time polymerase chain reaction (PCR) on a single cartridge. Extraction of genomic material was automated by manipulating magnetic particles in droplets using a series of planar coil electromagnets assisted by topographical features, enabling efficient fluidic processing over a variety of buffers and reagents. The functionality of the platform was demonstrated by performing nucleic acid extraction from whole blood, followed by real-time PCR detection of KRAS oncogene. Automated sample processing from whole blood to PCR-ready droplet was performed in 15 minutes. We took a modular approach of decoupling the modules of magnetic manipulation and optical detection from the device itself, enabling a low-complexity cartridge that operates in tandem with simple external instruments.
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