Revealing the role of nitroxyl during hepatic ischemia-reperfusion injury with a NIR-II luminescent nanoprobe.
Revealing the role of nitroxyl during hepatic ischemia-reperfusion injury with a NIR-II luminescent nanoprobe.
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DOI：
10.1039/d3sc02338a
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发表时间：
2023-07-19
期刊：
影响因子：
8.4
通讯作者：
Liu, Zhihong
中科院分区：
文献类型：
--
作者：
Li, Chenchen;Bi, Wenqiang;Liang, Tao;Li, Zhen;Liu, Zhihong
肝缺血再灌注损伤（HIRI）可严重损害肝功能，与活性氮有潜在的关系。硝酰基（Nitroxyl，HNO）具有多种生物学功能和药理活性。然而，到目前为止，还没有关于HNO在HIRI过程中的作用的知识，主要是因为在体内分子水平上准确跟踪其波动是极其困难的。在此，我们开发了一种响应性比率近红外-II（NIR-II）纳米探针，其具有稀土离子掺杂的纳米颗粒（RENP）作为发光体和分子触发剂，其可以特异性地与HNO反应以调节RENP的NIR-II发射。利用这种纳米探针，我们揭示了HNO和HIRI过程之间的关系，并证明HNO可能是HIRI过程中应激反应的产物。这项工作不仅创造了一个有用的工具，在体内视觉跟踪HNO，但也提供了第一手资料，它在HIRI的作用。开发了一种可活化的比率NIR-II纳米探针，以可视化体内HIRI过程中HNO水平的波动。利用该探针，我们揭示了HNO作为HIRI过程中应激反应产物的生理作用。
Hepatic ischemia-reperfusion injury (HIRI) can severely impair liver function and has a potential relationship with reactive nitrogen species. Nitroxyl (HNO) has been discovered to be involved in some biological functions and pharmacological activities. However, till now, there has been no knowledge of the role of HNO in the HIRI process, mainly because accurately tracking its fluctuation at the molecular level in vivo is extremely difficult. Herein, we developed a responsive ratiometric near-infrared-II (NIR-II) nanoprobe with rare earth ions-doped nanoparticles (RENPs) as the luminophore and a molecular trigger that can specifically react with HNO to regulate the NIR-II emission of RENPs. With this nanoprobe, we revealed the relationship between HNO and the HIRI process and demonstrated that HNO may be a product of stress reactions during HIRI. This work not only creates a useful tool for visually tracking HNO in vivo but also provides first-hand information about its role in HIRI. An activatable ratiometric NIR-II nanoprobe was developed to visualize the fluctuation of HNO levels in the HIRI process in vivo. With this probe, we reveal the physiological role of HNO as a product of stress reaction during the HIRI process.
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DOI：
10.4018/ijmdem.2021040101
发表时间：
2021-04-01
影响因子：
0.9
作者：
Chen, Jing;Li, Haifeng;Bo, Hongjian
通讯作者：
Bo, Hongjian
影响因子：
15.3
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影响因子：
14
作者：
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Chen, Lingxin
影响因子：
7.4
作者：
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通讯作者：
Tang, Bo