Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform.
Highly sensitive and ultra-rapid antigen-based detection of SARS-CoV-2 using nanomechanical sensor platform.
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DOI：
10.1016/j.bios.2021.113647
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发表时间：
2022-01-01
影响因子：
12.6
通讯作者：
Dravid VP
中科院分区：
文献类型：
--
作者：
Agarwal DK;Nandwana V;Henrich SE;Josyula VPVN;Thaxton CS;Qi C;Simons LM;Hultquist JF;Ozer EA;Shekhawat GS;Dravid VP
新冠肺炎的迅速传播，包括最近出现的具有极端病理范围的新变种，产生了迫切需要开发一种通用传感器来快速、准确和高灵敏地检测SARS-CoV-2。在这里，我们报道了一种基于微悬臂梁的光学检测SARS-CoV-2抗原蛋白的方法，该方法利用流体原子力显微镜(f-AFM)介导的纳米机械偏转方法，在短短几分钟内即可检测到SARS-CoV-2抗原蛋白。针对靶抗原的相应抗体首先被嫁接到经过EDC-NHS化学预官能化的镀金微悬臂梁表面，以实现合适的抗体-抗原相互作用。通过实时监测抗体-抗原相互作用诱导的纳米机械信号，首次证明了在临床相关浓度下(33 PM)快速检测SARS-CoV-2核衣壳(N)和刺突(S1)受体结合域(RBD)蛋白。更重要的是，我们进一步展示了用qRT-PCR预先确定的患者的鼻咽拭子样本中具有高特异性的抗原检测。结果不到5分钟(拭子到信号≤5分钟)，并显示出高选择性和分析灵敏度(LOD：100拷贝/毫升；0.71毫微克/毫升N蛋白)。这些发现证明了纳米机械信号转导技术在早期筛选SARS-CoV-2及其相关突变体的快速抗原检测方面的潜力。
The rapid spread of COVID-19 including recent emergence of new variants with its extreme range of pathologies create an urgent need to develop a versatile sensor for a rapid, precise, and highly sensitive detection of SARS-CoV-2. Herein, we report a microcantilever-based optical detection of SARS-CoV-2 antigenic proteins in just few minutes with high specificity by employing fluidic-atomic force microscopy (f-AFM) mediated nanomechanical deflection method. The corresponding antibodies against the target antigens were first grafted on the gold-coated microcantilever surface pre-functionalized with EDC-NHS chemistry for a suitable antibody-antigen interaction. Rapid detection of SARS-CoV-2 nucleocapsid (N) and spike (S1) receptor binding domain (RBD) proteins was first demonstrated at a clinically relevant concentration down to 1 ng/mL (33 pM) by real-time monitoring of nanomechanical signal induced by antibody-antigen interaction. More importantly, we further show high specific detection of antigens with nasopharyngeal swab specimens from patients pre-determined with qRT-PCR. The results take less than 5 min (swab to signal ≤5 min) and exhibit high selectivity and analytical sensitivity (LoD: 100 copies/ ml; 0.71 ng/ml of N protein). These findings demonstrate potential for nanomechanical signal transduction towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants.
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DOI：
10.1016/j.bbrc.2020.11.026
发表时间：
2021-01-29
影响因子：
3.1
作者：
Jackson CB;Zhang L;Farzan M;Choe H
通讯作者：
Choe H
影响因子：
3.1
作者：
Burton J;Love H;Richards K;Burton C;Summers S;Pitman J;Easterbrook L;Davies K;Spencer P;Killip M;Cane P;Bruce C;Roberts ADG
通讯作者：
Roberts ADG
影响因子：
56.9
作者：
Fritz, J;Baller, MK;Gimzewski, JK
通讯作者：
Gimzewski, JK
影响因子：
7.8
作者：
Gómez CE;Perdiguero B;Esteban M
通讯作者：
Esteban M
影响因子：
9.4
作者：
Basu, Atreyee;Zinger, Tatyana;Aguero-Rosenfeld, Maria E.
通讯作者：
Aguero-Rosenfeld, Maria E.