The auriculo-vagal afferent pathway and its role in seizure suppression in rats.
The auriculo-vagal afferent pathway and its role in seizure suppression in rats.
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耳迷走神经传入通路及其在抑制大鼠癫痫发作中的作用
DOI：
10.1186/1471-2202-14-85
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发表时间：
2013-08-09
期刊：
影响因子：
2.4
通讯作者：
Ben H
中科院分区：
文献类型：
--
作者：
He W;Jing XH;Zhu B;Zhu XL;Li L;Bai WZ;Ben H
从迷走神经耳支（ABVN）到孤束核（NTS）的传入投射被认为是耳-迷走反射中副交感神经张力增加的解剖学基础。NTS作为迷走神经的传入中枢，被认为在颈迷走神经刺激（VNS）的抗惊厥作用中起作用。本研究提出了一种“耳-迷走神经传入通路”（AVAP），经皮耳迷走神经刺激（ta-VNS）通过激活NTS神经元来抑制戊四唑（PTZ）诱导的大鼠癫痫发作。首次在大鼠身上观察到ABVN向NTS的传入投射。ta-VNS增加了清醒大鼠癫痫发作的第一次大发作潜伏期，降低了癫痫发作评分。此外，当NTS神经元放电频率降低时，麻醉大鼠的癫痫样活动表现为脑电图（EEG）同步增加，延迟0.37±0.12 s。NTS神经元的瞬时频率、平均频率的变化与脑电迹线的癫痫活动幅度呈负相关。ta-VNS通过增加NTS神经元的放电率显著抑制脑电图痕迹中的癫痫样活动。与tan-VNS相比，VNS和ta-VNS的抗惊厥持续时间显著延长（P<0.01）。VNS与ta-VNS抗惊厥持续时间差异无统计学意义（P < 0.05）。ta-VNS的抗惊厥作用因NTS的可逆冷阻断而减弱。ABVN和NTS之间存在解剖关系，这有力地支持了ta-VNS通过AVAP抑制大鼠癫痫样活动的可能性。ta-VNS将为神经系统疾病提供替代治疗方法，避免VNS的缺点。
The afferent projections from the auricular branch of the vagus nerve (ABVN) to the nucleus tractus solitaries (NTS) have been proposed as the anatomical basis for the increased parasympathetic tone seen in auriculo-vagal reflexes. As the afferent center of the vagus nerve, the NTS has been considered to play roles in the anticonvulsant effect of cervical vagus nerve stimulation (VNS). Here we proposed an “auriculo-vagal afferent pathway” (AVAP), by which transcutaneous auricular vagus nerve stimulation (ta-VNS) suppresses pentylenetetrazol (PTZ)-induced epileptic seizures by activating the NTS neurons in rats. The afferent projections from the ABVN to the NTS were firstly observed in rats. ta-VNS increased the first grand mal latency of the epileptic seizure and decreased the seizure scores in awake rats. Furthermore, when the firing rates of the NTS neurons decreased, epileptiform activity manifested as electroencephalogram (EEG) synchronization increased with 0.37±0.12 s delay in anaesthetized rats. The change of instantaneous frequency, mean frequency of the NTS neurons was negative correlated with the amplitude of the epileptic activity in EEG traces. ta-VNS significantly suppressed epileptiform activity in EEG traces via increasing the firing rates of the neurons of the NTS. In comparison with tan-VNS, the anticonvulsant durations of VNS and ta-VNS were significantly longer (P<0.01). There was no significant difference between the anticonvulsant durations of VNS and ta-VNS (P>0.05). The anticonvulsant effect of ta-VNS was weakened by reversible cold block of the NTS. There existed an anatomical relationship between the ABVN and the NTS, which strongly supports the concept that ta-VNS has the potential for suppressing epileptiform activity via the AVAP in rats. ta-VNS will provide alternative treatments for neurological disorders, which can avoid the disadvantage of VNS.
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影响因子：
1.4
作者：
BLOUSTINE, S;LANGSTON, L;MILLER, T
通讯作者：
MILLER, T
DOI：
10.1016/j.cnr.2004.06.007
发表时间：
2004-07-01
期刊：
CLINICAL NEUROSCIENCE RESEARCH
影响因子：
--
作者：
Labar, D
通讯作者：
Labar, D
影响因子：
5.6
作者：
Magdaleno-Madrigal, VM;Valdés-Cruz, A;Fernández-Guardiola, A
通讯作者：
Fernández-Guardiola, A
影响因子：
5.6
作者：
Schachter, SC;Saper, CB
通讯作者：
Saper, CB
影响因子：
1.9
作者：
Barberio, Michael;Reiter, Pamela D.;Dobyns, Emily L.
通讯作者：
Dobyns, Emily L.