Global proteomic analyses of human cytotrophoblast differentiation/invasion.
Global proteomic analyses of human cytotrophoblast differentiation/invasion.
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DOI：
10.1242/dev.199561
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发表时间：
2021-07-01
期刊：
影响因子：
--
通讯作者：
Fisher SJ
中科院分区：
文献类型：
--
作者：
Chen H;Williams KE;Kwan EY;Kapidzic M;Puckett KA;Aburajab RK;Robinson JF;Fisher SJ
在人类怀孕期间，来自胎盘的细胞滋养细胞（CTBs）分化成专门的亚群，在胎儿的正常生长和发育中起着至关重要的作用。这些CTBs的一个子集沿着侵入性途径分化，穿透蜕膜并将胎盘固定在子宫上。怀孕的一个关键障碍是这些细胞迁移、侵入和重塑螺旋动脉的能力，以确保足够的血液流动来滋养发育中的胎儿。尽管在描述调节这些细胞分化的分子特征方面取得了进展，但在妊娠中期对其整体蛋白质组学变化的评估仍不明确。在这里，使用所有理论片段离子谱的顺序窗口采集（SWATH），这是一种独立于数据的采集策略，我们表征了妊娠中期人类CTBs在向侵袭性表型分化过程中的蛋白质库。这种基于质谱的方法可以在四个培养时间点上识别3026种蛋白质，这些时间点对应于分化的顺序阶段，确认了已建立分子的表达动态，并为其他相关途径提供了新的信息。SWATH CTB全球光谱库的可用性为假设生成提供了有益的资源，并为进一步了解CTB分化动态奠定了基础。摘要：人类妊娠中期原代绒毛细胞滋养层细胞沿侵袭性途径分化的公开可用的SWATH-MS全球谱库的生成。
During human pregnancy, cytotrophoblasts (CTBs) from the placenta differentiate into specialized subpopulations that play crucial roles in proper fetal growth and development. A subset of these CTBs differentiate along an invasive pathway, penetrating the decidua and anchoring the placenta to the uterus. A crucial hurdle in pregnancy is the ability of these cells to migrate, invade and remodel spiral arteries, ensuring adequate blood flow to nourish the developing fetus. Although advances continue in describing the molecular features regulating the differentiation of these cells, assessment of their global proteomic changes at mid-gestation remain undefined. Here, using sequential window acquisition of all theoretical fragment-ion spectra (SWATH), which is a data-independent acquisition strategy, we characterized the protein repertoire of second trimester human CTBs during their differentiation towards an invasive phenotype. This mass spectrometry-based approach allowed identification of 3026 proteins across four culture time points corresponding to sequential stages of differentiation, confirming the expression dynamics of established molecules and offering new information into other pathways involved. The availability of a SWATH CTB global spectral library serves as a beneficial resource for hypothesis generation and as a foundation for further understanding CTB differentiation dynamics. Summary: The generation of a publicly available SWATH-MS global spectral library of human second-trimester primary villous cytotrophoblasts differentiating along an invasive pathway.
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作者：
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DOI：
10.3109/14767058.2012.733764
发表时间：
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