Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5
Crossover Formation During Rice Meiosis Relies on Interaction of OsMSH4 and OsMSH5
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水稻减数分裂过程中交叉的形成依赖于 OsMSH4 和 OsMSH5 的相互作用。
DOI：
10.1534/genetics.114.168732
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发表时间：
2014-10
期刊：
影响因子：
3.3
通讯作者：
Zhukuan Cheng
中科院分区：
文献类型：
--
作者：
Lei Zhang;Ding Tang;Qiong Luo;Xiaojun Chen;Hongjun Wang;Yafei Li;Zhukuan Cheng
MSH 4编码一种MutS蛋白，在减数分裂中发挥特殊作用。在真核生物中，如芽殖酵母、小鼠、秀丽隐杆线虫和拟南芥，msh 4突变体表现出减数分裂缺陷，交叉数目减少。本研究利用图位克隆技术对水稻MSH 4基因进行了鉴定。在Osmsh 4突变体中，交叉频率显著降低至野生型的10%左右，但联会复合体正常安装。双突变体分析表明，Osmsh 4 Osmsh 5突变体的交叉减少程度大于其它zmm突变体。这与OsZIP 4和OsMER 3在Osmsh 4中不存在定位一致，并且表明OsMSH 4和OsMSH 5比其他ZMM蛋白更早地发挥作用，其中可能需要它们来稳定祖细胞Holliday连接。利用酵母双杂交和pull-down实验，首次证实了OsMSH 4和OsMSH 5以及OsMSH 5和HEI 10在植物中的直接物理关联。已经在哺乳动物中证明了MSH 4-MSH 5异二聚体稳定祖细胞和双霍利迪连接的形成，其可以作为交叉（CO）被解析。我们认为OsMSH 4与OsMSH 5相互作用促进了水稻中大部分CO的形成。
MSH4encodes a MutS protein that plays a specialized role in meiosis. In eukaryotic species, such as budding yeast, mice,Caenorhabditis elegans, andArabidopsis,msh4mutants display meiotic defects with a reduced number of chiasmata. Here, we characterized riceMSH4by map-based cloning. InOsmsh4mutants, the chiasma frequency was dramatically decreased to ∼10% of the wild type, but the synaptonemal complex was normally installed. The double mutant analysis showed that in theOsmsh4 Osmsh5mutant, the reduction of chiasmata was greater than otherzmmmutants. This was consistent with the absence of localization for OsZIP4 and OsMER3 inOsmsh4and suggests an earlier role for OsMSH4 and OsMSH5 than other ZMM proteins where they may be required to stabilize progenitor Holliday junctions. Using yeast two-hybrid and pull-down assays, we verified the direct physical association between OsMSH4 and OsMSH5 and OsMSH5 and HEI10 in plants for the first time. The MSH4–MSH5 heterodimer has been demonstrated in mammals to stabilize the formation of progenitor and double Holliday junctions that may be resolved as crossovers (COs). We propose that OsMSH4 interacts with OsMSH5 to promote formation of the majority of COs in rice.
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影响因子：
64.5
作者：
M. Sym;G. Roeder
通讯作者：
M. Sym;G. Roeder
DOI：
10.1074/jbc.m104003200
发表时间：
2001-08
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
T. Nakagawa;H. Flores-Rozas;R. Kolodner
通讯作者：
T. Nakagawa;H. Flores-Rozas;R. Kolodner
影响因子：
64.5
作者：
Schwacha, A;Kleckner, N
通讯作者：
Kleckner, N
影响因子：
3.5
作者：
Santucci-Darmanin, S;Neyton, S;Paquis-Flucklinger, V
通讯作者：
Paquis-Flucklinger, V
影响因子：
2.6
作者：
Moran, ES;Armstrong, SJ;Jones, GH
通讯作者：
Jones, GH