Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones.
Tracing phylogenomic events leading to diversity of Haemophilus influenzae and the emergence of Brazilian Purpuric Fever (BPF)-associated clones.
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DOI：
10.1016/j.ygeno.2010.07.005
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发表时间：
2010-11
期刊：
影响因子：
4.4
通讯作者：
Peterson, Scott N.
中科院分区：
文献类型：
--
作者：
Papazisi, Leka;Ratnayake, Shashikala;Remortel, Brian G.;Bock, Geoffrey R.;Liang, Wei;Saeed, Alexander I.;Liu, Jia;Fleischmann, Robert D.;Kilian, Mogens;Peterson, Scott N.
在这里，我们报告了使用多基因组DNA微阵列来阐明与引起巴西紫癜热（BPF）的流感嗜血杆菌生物群埃及伊蚊克隆变异出现相关的基因组事件，巴西紫癜热（BPF）是一种具有高死亡率的重要儿科疾病。我们对菌株HK1212独特位点进行定向基因组测序，构建物种DNA微阵列。使用这种微阵列的比较基因组杂交使我们能够确定和比较基因互补，并推断出物种成员之间可靠的系统基因组关系。在bpf相关菌株（克隆）及其近亲的基因组中观察到较高的基因组变异性，其特征可能是与一些功能角色类别相关的显著基因通量。我们发现获得大量具有许多细胞膜蛋白的毒力决定因子，与参与转运、中心生物合成途径，特别是能量产生途径的基因的缺失相结合，是BPF基因组变异体的特征。
Here we report the use of a multi-genome DNA microarray to elucidate the genomic events associated with the emergence of the clonal variants of H. influenzae biogroup aegyptius causing Brazilian Purpuric Fever (BPF), an important pediatric disease with a high mortality rate. We performed directed genome sequencing of strain HK1212 unique loci to construct a species DNA microarray. Comparative genome hybridization using this microarray enabled us to determine and compare gene complements, and infer reliable phylogenomic relationships among members of the species. The higher genomic variability observed in the genomes of BPF-related strains (clones) and their close relatives may be characterized by significant gene flux related to a subset of functional role categories. We found that the acquisition of a large number of virulence determinants featuring numerous cell membrane proteins coupled to the loss of genes involved in transport, central biosynthetic pathways and in particular, energy production pathways to be characteristics of the BPF genomic variants.
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影响因子：
12.3
作者：
Hogg JS;Hu FZ;Janto B;Boissy R;Hayes J;Keefe R;Post JC;Ehrlich GD
通讯作者：
Ehrlich GD
DOI：
10.1073/pnas.0503252102
发表时间：
2005-11-01
影响因子：
11.1
作者：
Champion, OL;Gaunt, MW;Wren, BW
通讯作者：
Wren, BW
影响因子：
56.9
作者：
FLEISCHMANN, RD;ADAMS, MD;VENTER, JC
通讯作者：
VENTER, JC
影响因子：
3.1
作者：
Fernaays, Matthew M.;Lesse, Alan J.;Murphy, Timothy F.
通讯作者：
Murphy, Timothy F.
影响因子：
3.2
作者：
Davis, J;Smith, AL;Golomb, M
通讯作者：
Golomb, M