Quaternized chitosan nanoparticles loaded with the combined attenuated live vaccine against Newcastle disease and infectious bronchitis elicit immune response in chicken after intranasal administration.
Quaternized chitosan nanoparticles loaded with the combined attenuated live vaccine against Newcastle disease and infectious bronchitis elicit immune response in chicken after intranasal administration.
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季铵化壳聚糖纳米粒子负载新城疫和传染性支气管炎联合减毒活疫苗，鼻内给药后可引起鸡的免疫反应
DOI：
10.1080/10717544.2017.1388450
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发表时间：
2017-11
期刊：
影响因子：
6
通讯作者：
Jin Z
中科院分区：
文献类型：
--
作者：
Zhao K;Li S;Li W;Yu L;Duan X;Han J;Wang X;Jin Z
摘要 新城疫（ND）和传染性支气管炎（IB）是引起鸡呼吸道疾病的重要疾病，给养禽业造成严重的经济损失。本研究合成了N-2-羟丙基三甲基氯化铵壳聚糖（N-2-HACC）和N,O-羧甲基壳聚糖（CMC）作为疫苗抗原的佐剂和递送载体。制备了 N-2-HACC-CMC/NDV/IBV 纳米颗粒（NP）（NDV/La Sota 和 IBV/H120 封装在 N-2-HACC-CMC NP 中）和 N-2-HACC-CMC/NDV-IBV NP（N-2-HACC-CMC/NDV NP 和 N-2-HACC-CMC/IBV NP 以 1:1 的比例混合）分别采用聚电解质复合法。两种纳米颗粒均表现出较低的细胞毒性和较高的稳定性。当它们在37℃下储存三周时，它们的生物活性得以保持。体外释放测定表明，NDV 和 IBV 在初始爆发释放后都可以从纳米颗粒中持续释放。鸡体内免疫显示，N-2-HACC-CMC/NDV/IBV NPs或N-2-HACC-CMC/NDV-IBV NPs鼻内诱导产生更高滴度的IgG和IgA抗体，显着促进淋巴细胞增殖，并诱导比商业联合减毒活疫苗更高水平的白细胞介素-2（IL-2）、IL-4和干扰素-γ（IFN-γ）水平。这是动物疫苗领域的第一项研究，证明鸡鼻内给予壳聚糖衍生物封装的抗原（NDV和IBV）可以诱导体液、细胞和粘膜免疫反应，从而保护鸡免受高毒力NDV和IBV的感染。该研究表明，N-2-HACC-CMC可作为有效的佐剂和递送载体，用于进一步开发粘膜疫苗和药物，在医学上具有巨大的应用潜力。
Abstract Newcastle disease (ND) and infectious bronchitis (IB) are important diseases, which cause respiratory diseases in chickens, resulting in severely economic losses in the poultry industry. In this study, N-2-hydroxypropyl trimethyl ammonium chloride chitosan (N-2-HACC) and N,O-carboxymethyl chitosan (CMC) were synthesized as adjuvant and delivery carrier for vaccine antigens. N-2-HACC-CMC/NDV/IBV nanoparticles (NPs) (NDV/La Sota and IBV/H120 encapsulated in N-2-HACC-CMC NPs) and N-2-HACC-CMC/NDV-IBV NPs (the mixing of N-2-HACC-CMC/NDV NPs and N-2-HACC-CMC/IBV NPs in a ratio of 1:1) were prepared by the polyelectrolyte composite method, respectively. Both nanoparticles exhibited lower cytotoxicity and higher stability. Their bioactivities were maintained when they were stored at 37 °C for three weeks. Release assay in vitro showed that both NDV and IBV could be sustainably released from the nanoparticles after an initial burst release. In vivo immunization of chickens showed that N-2-HACC-CMC/NDV/IBV NPs or N-2-HACC-CMC/NDV-IBV NPs intranasally induced higher titers of IgG and IgA antibodies, significantly promoted proliferation of lymphocytes and induced higher levels of interleukine-2 (IL-2), IL-4 and interferon-γ (IFN-γ) than the commercially combined attenuated live vaccine did. This is the first study in the field of animal vaccines demonstrating that intranasal administration of chickens with antigens (NDV and IBV) encapsulated with chitosan derivative could induce humoral, cellular, and mucosal immune responses, which protected chickens from the infection of highly virulent NDV and IBV. This study indicated that N-2-HACC-CMC could be used as an efficient adjuvant and delivery carrier for further development of mucosal vaccines and drugs and could have an immense application potential in medicine.
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影响因子：
2.4
作者：
Kouakou AV;Kouakou V;Kouakou C;Godji P;Kouassi AL;Krou HA;Langeois Q;Webby RJ;Ducatez MF;Couacy-Hymann E
通讯作者：
Couacy-Hymann E
影响因子：
7.3
作者：
Jankovic D;Feng CG
通讯作者：
Feng CG
影响因子：
5.8
作者：
Gavini, E;Hegge, AB;Giunchedi, P
通讯作者：
Giunchedi, P
影响因子：
2.9
作者：
Kapczynski, Darrell R.;Afonso, Claudio L.;Miller, Patti J.
通讯作者：
Miller, Patti J.
DOI：
10.3109/21691401.2014.966193
发表时间：
2016-02-17
影响因子：
5.8
作者：
Arthanari, Saravanakumar;Mani, Ganesh;Jang, Hyun Tae
通讯作者：
Jang, Hyun Tae