Identification of glycoproteins containing specific glycans using a lectin-chemical method.
Identification of glycoproteins containing specific glycans using a lectin-chemical method.
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DOI：
10.1021/ac504304v
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发表时间：
2015
影响因子：
7.4
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
--
作者：
Li, Yan;Shah, Punit;De Marzo, Angelo M.;Van Eyk, Jennifer E.;Lo, Qianqian;Chan, Daniel W.;Zhang, Hui
糖基化是最常见的蛋白质修饰之一。每个糖蛋白可以在多个糖位点上糖基化，每个糖位点可以被不同的聚糖修饰。由于糖基化的异质性，研究特定聚糖及其影响的糖蛋白的结构-功能关系已被证明是困难的。在这里，我们报告了一种快速定量鉴定含有特定聚糖的糖蛋白的新方法。凝集素亲和分离之后，化学固定捕获的糖肽，允许随后的质谱鉴定含有特定聚糖的糖蛋白。该方法的应用应该有助于我们了解聚糖的变化与疾病的关系，并发现具有某些聚糖的新型糖蛋白，这些糖蛋白可以作为生物标志物或治疗靶点。
Glycosylation is one of the most common protein modifications. Each glycoprotein can be glycosylated at multiple glycosites, and each glycosites can be modified by different glycans. Due to this heterogeneity of glycosylation, it has proven difficult to study the structure–function relationship of specific glycans and their affected glycoproteins. Here, we report a novel method for rapid and quantitative identification of glycoproteins containing specific glycans. Lectin affinity isolations are followed by chemical immobilization of the captured glycopeptides, allowing the identification of glycoproteins containing specific glycans by subsequent mass spectrometry. The application of the method should be useful to facilitate our understanding of how changes in glycan associate with diseases, and to discover novel glycoproteins with certain glycans that could serve as biomarkers or therapeutic targets.
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