The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment.
The Skin Microbiome of Patients With Atopic Dermatitis Normalizes Gradually During Treatment.
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DOI：
10.3389/fcimb.2021.720674
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发表时间：
2021
影响因子：
5.7
通讯作者：
García-Romero MT
中科院分区：
文献类型：
--
作者：
Khadka VD;Key FM;Romo-González C;Martínez-Gayosso A;Campos-Cabrera BL;Gerónimo-Gallegos A;Lynn TC;Durán-McKinster C;Coria-Jiménez R;Lieberman TD;García-Romero MT
特应性皮炎（AD）的特征是皮肤微生物群的改变，主要由金黄色葡萄球菌定植。标准治疗包括润肤剂、抗炎药物和防腐剂。表征AD治疗期间皮肤微生物组的变化。在一项纵向前瞻性研究中，研究了中度至重度AD儿童和健康儿童的皮肤微生物组。AD患者被随机分为两组，一组接受润肤剂和局部皮质类固醇的标准治疗，另一组接受添加稀释漂白剂（DBB）的标准治疗，并在三个月内进行四次就诊取样。在每次访问时，测量AD的严重程度，从四个身体部位提取拭子，并使用16S rRNA扩增技术评估这些部位的微生物组组成。我们纳入了14名健康对照和28名患者。我们发现患者中金黄色葡萄球菌的相对丰度较高，这与AD的严重程度和明显α多样性降低有关。随着疾病严重程度的改善和治疗，金黄色葡萄球菌的丰度下降，逐渐变得更类似于健康对照的微生物组。治疗后，接受DBB治疗的患者的金黄色葡萄球菌丰度明显低于仅接受标准治疗的患者。健康对照者和阿尔茨海默病患者的皮肤微生物组有明显的差异，这种差异随着治疗而减少。三个月后，在标准治疗中加入DBB显著降低了金黄色葡萄球菌的负担，支持将其作为一种治疗选择。需要进一步的双盲试验研究。
Atopic dermatitis (AD) is characterized by an altered skin microbiome dominantly colonized by S. aureus. Standard treatment includes emollients, anti-inflammatory medications and antiseptics. To characterize changes in the skin microbiome during treatment for AD. The skin microbiomes of children with moderate-to-severe AD and healthy children were investigated in a longitudinal prospective study. Patients with AD were randomized to receive either standard treatment with emollients and topical corticosteroids or standard treatment with the addition of dilute bleach baths (DBB) and sampled at four visits over a three-month period. At each visit, severity of AD was measured, swabs were taken from four body sites and the composition of the microbiome at those sites was assessed using 16S rRNA amplification. We included 14 healthy controls and 28 patients. We found high relative abundances of S. aureus in patients, which correlated with AD severity and reduced apparent alpha diversity. As disease severity improved with treatment, the abundance of S. aureus decreased, gradually becoming more similar to the microbiomes of healthy controls. After treatment, patients who received DBB had a significantly lower abundance of S. aureus than those who received only standard treatment. There are clear differences in the skin microbiome of healthy controls and AD patients that diminish with treatment. After three months, the addition of DBB to standard treatment had significantly decreased the S. aureus burden, supporting its use as a therapeutic option. Further study in double-blinded trials is needed.
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影响因子：
4.5
作者：
Fritz SA;Camins BC;Eisenstein KA;Fritz JM;Epplin EK;Burnham CA;Dukes J;Storch GA
通讯作者：
Storch GA
影响因子：
10.3
作者：
Harkins, C. P.;McAleer, M. A.;Holden, M. T. G.
通讯作者：
Holden, M. T. G.
影响因子：
15.9
作者：
Leung, Thomas H.;Zhang, Lillian F.;Kim, Seung K.
通讯作者：
Kim, Seung K.
儿童特应性皮炎的氟替卡松乳膏和辅助漂白浴的皮肤微生物组作用。
影响因子：
13.8
作者：
Gonzalez ME;Schaffer JV;Orlow SJ;Gao Z;Li H;Alekseyenko AV;Blaser MJ
通讯作者：
Blaser MJ
影响因子：
4.6
作者：
Iwamoto, Kazumasa;Moriwaki, Masaya;Hide, Michihiro
通讯作者：
Hide, Michihiro