Melatonin prevents acute kidney injury in severely burned rats via the activation of SIRT1.
Melatonin prevents acute kidney injury in severely burned rats via the activation of SIRT1.
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褪黑素通过激活 SIRT1 预防严重烧伤大鼠的急性肾损伤
DOI：
10.1038/srep32199
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发表时间：
2016-09-07
影响因子：
4.6
通讯作者：
Hu DH
中科院分区：
文献类型：
--
作者：
Bai XZ;He T;Gao JX;Liu Y;Liu JQ;Han SC;Li Y;Shi JH;Han JT;Tao K;Xie ST;Wang HT;Hu DH
急性肾损伤（AKI）是严重烧伤后的常见并发症。据报道，褪黑素可通过增加沉默信息调节因子1（SIRT1）的表达来保护机体免受多器官损伤，SIRT1可调节应激反应、炎症、细胞衰老和凋亡。本研究旨在探讨褪黑素对烧伤大鼠肾组织的保护作用以及SIRT1在其中所起的作用。建立大鼠严重烧伤模型，分别给予或不给予褪黑素和SIRT1抑制剂。通过检测肾功能和组织学表现来评估肾损伤的严重程度。分析乙酰化p53（Ac - p53）、乙酰化p65（Ac - p65）、核因子κB（NF - κB）、乙酰化叉头框蛋白O1（Ac - FoxO1）、Bcl - 2和Bax的水平以研究潜在机制。我们的研究结果表明，严重烧伤可诱发急性肾损伤，褪黑素可部分逆转这种损伤。褪黑素可减轻氧化应激、炎症和凋亡，同时伴有SIRT1表达增加。抑制SIRT1可消除褪黑素的保护作用。总之，我们证明褪黑素通过激活SIRT1信号通路改善严重烧伤诱导的AKI。
Acute kidney injury (AKI) is a common complication after severe burns. Melatonin has been reported to protect against multiple organ injuries by increasing the expression of SIRT1, a silent information regulator that regulates stress responses, inflammation, cellular senescence and apoptosis. This study aimed to investigate the protective effects of melatonin on renal tissues of burned rats and the role of SIRT1 involving the effects. Rat severely burned model was established, with or without the administration of melatonin and SIRT1 inhibitor. The renal function and histological manifestations were determined to evaluate the severity of kidney injury. The levels of acetylated-p53 (Ac-p53), acetylated-p65 (Ac-p65), NF-κB, acetylated-forkhead box O1 (Ac-FoxO1), Bcl-2 and Bax were analyzed to study the underlying mechanisms. Our results suggested that severe burns could induce acute kidney injury, which could be partially reversed by melatonin. Melatonin attenuated oxidative stress, inflammation and apoptosis accompanied by the increased expression of SIRT1. The protective effects of melatonin were abrogated by the inhibition of SIRT1. In conclusion, we demonstrate that melatonin improves severe burn-induced AKI via the activation of SIRT1 signaling.
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影响因子：
7.4
作者：
Guo SX;Fang Q;You CG;Jin YY;Wang XG;Hu XL;Han CM
通讯作者：
Han CM
影响因子：
4.6
作者：
Bai X;Fan L;He T;Jia W;Yang L;Zhang J;Liu Y;Shi J;Su L;Hu D
通讯作者：
Hu D
影响因子：
10.3
作者：
Chen, Hong-Hwa;Lin, Kun-Chen;Yip, Hon-Kan
通讯作者：
Yip, Hon-Kan
影响因子：
6.1
作者：
Hsing, Chung-Hsi;Chou, Willy;Yeh, Ching-Hua
通讯作者：
Yeh, Ching-Hua
影响因子：
8.2
作者：
Kim, M. Y.;Lim, J. H.;Park, C. W.
通讯作者：
Park, C. W.