Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation.
Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation.
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富氢盐水通过抑制氧化应激诱导的细胞凋亡和炎症对严重烧伤大鼠早期急性肾损伤的影响
DOI：
10.1186/s12967-015-0548-3
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发表时间：
2015-06-06
影响因子：
7.4
通讯作者：
Han CM
中科院分区：
文献类型：
--
作者：
Guo SX;Fang Q;You CG;Jin YY;Wang XG;Hu XL;Han CM
严重烧伤患者的早期急性肾损伤（阿基）预示着多因素的高死亡率。据报道，氢通过选择性淬灭活性氧来减轻器官损伤。本研究探讨了氢气对大鼠严重烧伤诱导的早期阿基的潜在保护作用。通过将大鼠剃毛的背部浸入100°C浴中15秒来诱导严重烧伤。将五十六只Sprague-Dawley大鼠随机分为假手术组、烧伤+生理盐水组和烧伤+富氢生理盐水组，测定肾功能和细胞凋亡指数。采用肾组织病理学、免疫荧光染色、实时荧光定量PCR、ELISA和Western blotting等方法，对烧伤后不同时间点的大鼠血清和肾组织进行检测，以探讨烧伤后不同时间点的影响及其机制。HS治疗可改善肾功能和肾小管细胞凋亡。此外，氧化还原电位和丙二醛水平显着降低与HS处理，而内源抗氧化酶活性显着增加。HS还降低了烧伤大鼠血清和肾组织中髓过氧化物酶水平，并影响炎症介质的释放。HS的调节作用包括抑制p38、JNK、ERK和NF-κB的活化，增加Akt的磷酸化。氢可以减轻严重烧伤诱导的早期阿基;其保护机制包括抑制氧化应激诱导的细胞凋亡和炎症，这可能是通过调节MAPK、Akt和NF-κB信号通路介导的。
Early acute kidney injury (AKI) in severely burned patients predicts a high mortality that is multi-factorial. Hydrogen has been reported to alleviate organ injury via selective quenching of reactive oxygen species. This study investigated the potential protective effects of hydrogen against severe burn-induced early AKI in rats. Severe burn were induced via immersing the shaved back of rats into a 100°C bath for 15 s. Fifty-six Sprague–Dawley rats were randomly divided into Sham, Burn + saline, and Burn + hydrogen-rich saline (HS) groups, and renal function and the apoptotic index were measured. Kidney histopathology and immunofluorescence staining, quantitative real-time PCR, ELISA and western blotting were performed on the sera or renal tissues of burned rats to explore the underlying effects and mechanisms at varying time points post burn. Renal function and tubular apoptosis were improved by HS treatment. In addition, the oxidation–reduction potential and malondialdehyde levels were markedly reduced with HS treatment, whereas endogenous antioxidant enzyme activities were significantly increased. HS also decreased the myeloperoxidase levels and influenced the release of inflammatory mediators in the sera and renal tissues of the burned rats. The regulatory effects of HS included the inhibition of p38, JNK, ERK and NF-κB activation, and an increase in Akt phosphorylation. Hydrogen can attenuate severe burn-induced early AKI; the mechanisms of protection include the inhibition of oxidative stress induced apoptosis and inflammation, which may be mediated by regulation of the MAPKs, Akt and NF-κB signalling pathways.
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富含氢的盐水对大鼠早期烧伤进展的有益影响。
影响因子：
3.7
作者：
Guo SX;Jin YY;Fang Q;You CG;Wang XG;Hu XL;Han CM
通讯作者：
Han CM
DOI：
10.1155/2011/768512
发表时间：
2011
期刊：
Journal of signal transduction
影响因子：
--
作者：
Feliers D;Kasinath BS
通讯作者：
Kasinath BS
DOI：
10.1016/j.bbrc.2008.05.165
发表时间：
2008-08-15
影响因子：
3.1
作者：
Hayashida, Kentaro;Sano, Motoaki;Fukuda, Keiichi
通讯作者：
Fukuda, Keiichi
DOI：
10.1016/j.bbrc.2007.07.088
发表时间：
2007-09-28
影响因子：
3.1
作者：
Fukuda, Kei-Ichi;Asoh, Sadamitsu;Ohta, Shigeo
通讯作者：
Ohta, Shigeo
影响因子：
3.8
作者：
Hoesel, Laszlo M.;Mattar, Aladdein F.;Hemmila, Mark R.
通讯作者：
Hemmila, Mark R.