Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.
复制标题
组蛋白标记阅读器 MRG1/2 通过与 CONSTANS 相互作用调节 FT 表达来调节拟南芥开花
DOI：
10.1371/journal.pgen.1004617
复制
发表时间：
2014-09
期刊：
影响因子：
4.5
通讯作者：
Dong AW
中科院分区：
文献类型：
--
作者：
Bu Z;Yu Y;Li Z;Liu Y;Jiang W;Huang Y;Dong AW
日长对于调节植物向生殖发育（开花）的过渡很重要。在模式植物拟南芥（Arabidopsis thaliana）中，转录因子CONSTANS（CO）促进成花基因FLOWERLOCUS T（FT）的表达，构成长日照光周期下的关键开花途径。近年来的研究表明，FT的表达受FT染色质组蛋白修饰标记的改变所调控，但与CO蛋白直接相互作用的表观遗传调控因子尚未被确定。在这里，我们表明，拟南芥Morf相关基因（MRG）组蛋白MRG 1和MRG 2作为H3 K4 me 3/H3 K36 me 3的读者和物理相互作用与CO激活FT的表达。体外结合分析表明，MRG 1和MRG 2的chromodomains优先结合H3 K4 me 3/H3 K36 me 3肽。的mrg 1 mrg 2双突变体表现出降低的mRNA水平的FT，但不CO，并显示出晚开花表型下的长日照，但不是短日照的光周期生长条件。MRG 2与FT启动子染色质的结合依赖于CO和H3 K4 me 3/H3 K36 me 3。反之亦然，MRG 1和MRG 2的缺失也会损害FT启动子处的CO结合。与H3 K4 me 3/H3 K36 me 3肽结合的MRG 2的晶体结构分析以及植物中的诱变分析进一步证明MRG 2功能依赖于其H3 K4 me 3/H3 K36 me 3结合活性。总的来说，我们的研究结果揭示了一种新的染色质调控机制，连接功能的MRG 1和MRG 2蛋白，H3 K4/H3 K36甲基化，和CO在FT激活光周期调节植物开花时间。
Day-length is important for regulating the transition to reproductive development (flowering) in plants. In the model plant Arabidopsis thaliana, the transcription factor CONSTANS (CO) promotes expression of the florigen FLOWERING LOCUS T (FT), constituting a key flowering pathway under long-day photoperiods. Recent studies have revealed that FT expression is regulated by changes of histone modification marks of the FT chromatin, but the epigenetic regulators that directly interact with the CO protein have not been identified. Here, we show that the Arabidopsis Morf Related Gene (MRG) group proteins MRG1 and MRG2 act as H3K4me3/H3K36me3 readers and physically interact with CO to activate FT expression. In vitro binding analyses indicated that the chromodomains of MRG1 and MRG2 preferentially bind H3K4me3/H3K36me3 peptides. The mrg1 mrg2 double mutant exhibits reduced mRNA levels of FT, but not of CO, and shows a late-flowering phenotype under the long-day but not short-day photoperiod growth conditions. MRG2 associates with the chromatin of FT promoter in a way dependent of both CO and H3K4me3/H3K36me3. Vice versa, loss of MRG1 and MRG2 also impairs CO binding at the FT promoter. Crystal structure analyses of MRG2 bound with H3K4me3/H3K36me3 peptides together with mutagenesis analysis in planta further demonstrated that MRG2 function relies on its H3K4me3/H3K36me3-binding activity. Collectively, our results unravel a novel chromatin regulatory mechanism, linking functions of MRG1 and MRG2 proteins, H3K4/H3K36 methylations, and CO in FT activation in the photoperiodic regulation of flowering time in plants.
登录
查看更多内容
影响因子：
11.8
作者：
Li, Dan;Liu, Chang;Yu, Hao
通讯作者：
Yu, Hao
影响因子：
11.4
作者：
Hoppmann, Verena;Thorstensen, Tage;Aasland, Rein
通讯作者：
Aasland, Rein
影响因子：
7.2
作者：
Lee, Woo Yong;Lee, Daeyoup;Kwon, Chang Seob
通讯作者：
Kwon, Chang Seob
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
4.8
作者：
Eisen, A;Utley, RT;Côté, J
通讯作者：
Côté, J