Immunohistochemical survey of cobalamin-binding proteins.
Immunohistochemical survey of cobalamin-binding proteins.
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钴胺素结合蛋白的免疫组织化学研究。
DOI：
10.1016/0016-5085(89)91687-9
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发表时间：
1989
期刊：
影响因子：
29.4
通讯作者：
DeSchryver-Kecskemeti,K
中科院分区：
文献类型：
--
作者：
Lee,EY;Seetharam,B;Alpers,DH;DeSchryver-Kecskemeti,K
膳食钴胺素与胃肠道内因子（IF）和非内因子（R蛋白）结合。先前仅在胃（壁细胞、主细胞）、十二指肠（粘液细胞）和胰液中描述了IF的分布。据报道含有R蛋白的细胞分布更为广泛，包括肠道的粘液分泌细胞。为了进一步阐明在不同器官中产生这些钴胺素结合物的细胞，我们使用间接免疫过氧化物酶组织化学方法，对四个物种的许多组织进行了调查，并对大鼠IF和猪R蛋白进行了单特异性抗血清培养。用纯化到均匀性的适当蛋白阻断后证实阳性染色模式。大鼠胃主细胞呈较强的IF染色，壁细胞染色较弱，但染色明显。内在因子仅在所有其他物种的壁细胞中被发现。R蛋白存在于所有被试物种的顶壁细胞中，除了猪的基底隐窝粘液细胞被染色。小肠上皮细胞的刷状边缘和顶端细胞质区域IF和R蛋白也呈阳性，可能反映了摄取。辅助消化器官，如唾液腺和胰腺分泌管中IF和R蛋白也呈阳性。辅助消化器官参与了IF和R蛋白的分泌，但这种IF分泌的生理重要性尚不清楚。
Dietary cobalamin is bound to intrinsic factor (IF) and nonintrinsic factor (R protein) in the gastrointestinal tract. Distribution of IF has previously been described only in the stomach (parietal, chief cells), duodenum (mucous cells), and pancreatic juice. Cells containing R protein have been reported to be more widespread, including mucin-secreting cells of the intestinal tract. To further clarify the cells producing these cobalamin binders in the various organs, we surveyed a number of tissues in four species, with monospecific antisera raised against rat IF and hog R protein, using indirect immunoperoxidase histochemistry. A positive staining pattern was confirmed after blocking with the appropriate protein purified to homogeneity. Strong IF staining was noted over chief cells in the rat stomach, with weaker but substantial staining in the parietal cells as well. Intrinsic factor was found only in parietal cells in all other species examined. R protein was found in parietal cells of all species tested, except in the hog, where mucous cells of the basal crypt stained. The area of the brush border and apical cytoplasm in small intestinal epithelial cells was also positive for both IF and R protein, probably reflecting uptake. Accessory digestive organs, such as salivary gland and pancreas were also positive for both IF and R protein in the secretory ducts. Accessory digestive organs contribute to the secretion of IF as well as R protein, but the physiologic importance of this IF secretion is not clear.
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分离的大鼠胃粘膜细胞的细胞起源和内因子释放。
DOI：
--
发表时间：
1983
期刊：
Biochimica et Biophysica Acta
影响因子：
--
作者：
W. Schepp;H. Ruoff;S. Miederer
通讯作者：
S. Miederer
血浆 R 结合剂缺乏和神经系统疾病。
影响因子：
158.5
作者：
Samuel H. Sigal;Charles A. Hall;Charles A. Hall;J. Antel;J. Antel
通讯作者：
J. Antel
维生素 B12 R 结合剂在正常人体组织中的分布：一项免疫组织化学研究。
影响因子：
3.2
作者：
H. Kudo;G. Ohshio;K. Ogawa;Y. Wakatsuki;M. Inada;Y. Hamashima;T. Miyake
通讯作者：
T. Miyake
人内在因子的免疫细胞化学定位：未刺激的胃。
影响因子：
29.4
作者：
J. Levine;P. Nakane;R. Allen
通讯作者：
R. Allen
与大鼠内因子复合的钴胺素的肠道摄取和释放。
DOI：
10.1152/ajpgi.1985.248.3.g326
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Seetharam,B;Presti,M;Frank,B;Tiruppathi,C;Alpers,DH
通讯作者：
Alpers,DH