Electron spin resonance spin label studies of plasma fibronectin: effect of temperature.
Electron spin resonance spin label studies of plasma fibronectin: effect of temperature.
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血浆纤连蛋白的电子自旋共振自旋标记研究：温度的影响。
DOI：
10.1016/0003-9861(84)90012-2
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发表时间：
1984
影响因子：
3.9
通讯作者：
Tooney,NM
中科院分区：
文献类型：
--
作者：
Lai,CS;Tooney,NM
Plasma fibronectin was chemically modified by 4-maleimido-2,2,6,6-tetramethylpi-peridinooxyl (maleimide spin label). Only the free sulfhydryl groups of plasma fibronectin were modified by the label under the experimental conditions. The ESR spectrum of spin-labeled fibronectin showed that the sites of labeling were highly immobilized, suggesting that the sulfhydryl groups of the protein are in small, confined environments. The conversion of the strongly immobilized ESR spectrum into a weakly immobilized one was observed when the spin-labeled protein was heated from 30 to 60 °C, indicating the thermal unfolding of the protein molecules. The midpoint temperature for the thermal unfolding of plasma fibronectin is about 50 °C. The results suggest that plasma fibronectin is stable to about 40 °C and starts unfolding above this temperature. The rotational correlation time estimated from the ESR spectrum of spin-labeled fibronectin at 21 °C was about 2.0 × 10−8s. The rotational correlation time calculated from the Stokes-Einstein equation, assuming a rigid globular configuration for fibronectin with a Stokes radius of 10 nm, was about 7.8 × 10−7s. The differences in rotational correlation time by a factor of 39 between experimental and calculated values do not support a globular configuration for plasma fibronectin.
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人血浆冷不溶性球蛋白的结构和稳定性。
影响因子：
4.8
作者：
S. S. Alexander;G. Colonna;H. Edelhoch
通讯作者：
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影响因子：
6.4
作者：
ENGVALL, E;RUOSLAHTI, E
通讯作者：
RUOSLAHTI, E
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DOI：
10.1016/0309-1651(77)90082-0
发表时间：
1977
期刊：
Cell biology international reports
影响因子：
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作者：
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通讯作者：
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DOI：
10.1016/0005-2736(79)90117-2
发表时间：
1979
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
F. Grinnell;D. Minter
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D. Minter
血浆和细胞纤连蛋白之间结构域的差异。
影响因子：
4.8
作者：
M. Hayashi;K. Yamada
通讯作者：
K. Yamada