Endothelial cell‑derived CCL15 mediates the transmigration of fibrocytes through the CCL15‑CCR1 axis in vitro.
Endothelial cell‑derived CCL15 mediates the transmigration of fibrocytes through the CCL15‑CCR1 axis in vitro.
复制标题
内皮细胞来源的CCL15在体外通过CCL15-CCR1轴介导纤维细胞的迁移。
DOI：
10.3892/mmr.2020.11610
复制
发表时间：
2020-12
影响因子：
3.4
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Pang N;Lin Z;Wang X;Xu L;Xu X;Huang R;Li X;Li X;Li J
伤口愈合是一个复杂的生理过程，其中纤维细胞起着至关重要的作用。然而，在伤口愈合过程中纤维细胞募集的机制在很大程度上仍然未知。本研究旨在探讨内皮细胞是否参与了伤口愈合过程中纤维细胞的募集。为了模拟体内血管生成过程，使用渗透性Transwell共培养系统实现了由内皮细胞和纤维细胞组成的共培养系统。然后用基因芯片测量内皮细胞共培养或不共培养产生的趋化因子的表达。基于芯片分析的数据集，内皮细胞产生的趋化因子配体15 （CCL15）被鉴定出来，可能在介导纤维细胞的转运中起调节作用。内皮细胞中过表达CCL15或纤维细胞中过表达趋化因子受体1 （chemokine receptor 1, CCR1）可促进纤维细胞的转运，而沉默内皮细胞中过表达CCL15或纤维细胞中过表达CCR1可减弱纤维细胞的转运。本研究结果表明，内皮细胞和纤维细胞之间的CCL15-CCR1轴在伤口愈合过程中介导纤维细胞的募集中起着至关重要的作用。
Wound healing is a complex physiological process in which fibrocytes serve a vital role. However, the mechanism underlying the recruitment of fibrocytes during wound healing remains largely unknown. The present study aimed to investigate whether endothelial cells are involved in the recruitment of fibrocytes in wound healing. To mimic the in vivo angiogenic process, a co-culture system consisting of endothelial cells and fibrocytes was achieved using a permeable Transwell co-culture system. The expression of chemokines produced by endothelial cells with or without co-culture was then measured using a gene chip. Based on the dataset from chip analysis, chemokine ligand 15 (CCL15) produced by endothelial cells was identified, which likely serves a regulatory role in mediating the transmigration of fibrocytes. Overexpression of CCL15 in endothelial cells or chemokine receptor 1 (CCR1) in fibrocytes promoted the transmigration of fibrocytes, whilst silencing the expression of CCL15 in endothelial cells or that of CCR1 in fibrocytes attenuated the transmigration of fibrocytes. Results from the present study suggested that the CCL15-CCR1 axis between endothelial cells and fibrocytes serves a vital role in mediating the recruitment of fibrocytes during wound healing.
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影响因子：
12.4
作者：
Singh SR;Sutcliffe A;Kaur D;Gupta S;Desai D;Saunders R;Brightling CE
通讯作者：
Brightling CE
影响因子：
2.9
作者：
Li Xueyong;Chen Shaozong;Li Jinqing
通讯作者：
Li Jinqing
影响因子：
4.4
作者：
Park, Keun Hyung;Lee, Tae Hoon;Kim, Jiyoung
通讯作者：
Kim, Jiyoung
影响因子：
4.6
作者：
Ishida Y;Kimura A;Nosaka M;Kuninaka Y;Hemmi H;Sasaki I;Kaisho T;Mukaida N;Kondo T
通讯作者：
Kondo T
影响因子：
8
作者：
Isgro, M.;Bianchetti, L.;Mattoli, S.
通讯作者：
Mattoli, S.