Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models.
Daikenchuto attenuates visceral pain and suppresses eosinophil infiltration in inflammatory bowel disease in murine models.
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DOI：
10.1002/jgh3.12410
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Dai Y
中科院分区：
文献类型：
--
作者：
Kogure Y;Kanda H;Wang S;Hao Y;Li J;Yamamoto S;Noguchi K;Dai Y
Daikenchuto (DKT) 是一种日本传统配方，由四种草药组成，用于治疗腹痛。炎症性肠病（IBD）包括溃疡性结肠炎（UC）和克罗恩病（CD），其特征是结肠炎症和慢性腹痛。本研究旨在探讨 DKT 是否能在动物模型中抑制与 IBD 相关的结肠过敏和炎症。 Sprague-Dawley大鼠结肠内注射4%右旋糖酐硫酸钠（DSS）或硫酸三硝基苯（TNBS），分别建立UC或CD模型。诱导结肠炎后第 3 至 7 天，每天口服一次 DKT 和 5-氨基水杨酸 (5-ASA)。第7天，通过测量对结直肠扩张（CRD）的内脏运动反应（VMR）和炎症指标（包括组织学评分、白细胞浸润长度、MPO活性和嗜酸性粒细胞计数）来评估内脏疼痛和炎症。 DSS 和 TNBS 增加了 CRD 的 VMR 和炎症指标。在 DSS 和 TNBS 治疗的大鼠中，DKT（而非 5-ASA）抑制了 VMR 至 CRD。 DKT 和 5-ASA 降低了两种 IBD 模型中的嗜酸性粒细胞计数。在 DSS 治疗的大鼠中，5-ASA（而非 DKT）抑制了 MPO 活性。在 TNBS 治疗的大鼠中，5-ASA 和 DKT 均不抑制 MPO 活性。这些结果表明 DKT 对 IBD 相关腹痛有益。 DKT 对 IBD 的抗炎作用可能与抑制嗜酸性粒细胞有关。 DKT 的抗炎作用机制与 5-ASA 部分不同。 DKT与常规药物的联合应用可能对IBD治疗产生积极的协同效应。据我们所知，我们首次证明，(i) daikenchuto (DKT) 可抑制与右旋糖酐硫酸钠或硫酸三硝基苯诱导的结肠炎相关的结肠过敏和嗜酸性粒细胞浸润。 (ii) DKT 的抗炎作用机制部分不同于 5-氨基水杨酸 (5-ASA)。 (iii) DKT和5-ASA的联合应用可能对IBD治疗产生积极的协同作用。
Daikenchuto (DKT), a traditional Japanese formula, comprises four herbal medicines and is used for abdominal pain. Inflammatory bowel disease (IBD) includes ulcerative colitis (UC) and Crohn's disease (CD) and is characterized by colonic inflammation and chronic abdominal pain. The present study aimed to investigate whether DKT suppresses colonic hypersensitivity and inflammation associated with IBD in animal models. Sprague–Dawley rats were administered 4% sodium dextran sulfate (DSS) or trinitrobenzene sulfate (TNBS) in the colon to establish UC or CD models, respectively. DKT and 5‐aminosalicylic acid (5‐ASA) were administered orally once a day from Days 3 to 7 after induction of colitis. On Day 7, visceral pain and inflammation were evaluated by measuring the visceromotor response (VMR) to colorectal distention (CRD) and inflammatory indicators, including histological score, length of leukocyte infiltration, MPO activity, and eosinophil count. DSS and TNBS increased VMR to CRD and the inflammation indicators. DKT, but not 5‐ASA, suppressed the VMR to CRD in DSS‐ and TNBS‐treated rats. DKT and 5‐ASA decreased the eosinophil count in both IBD models. In DSS‐treated rats, 5‐ASA, but not DKT, suppressed the MPO activity. In TNBS‐treated rats, neither 5‐ASA nor DKT suppressed MPO activity. These results suggest that DKT is beneficial for abdominal pain associated with IBD. The anti‐inflammatory effect of DKT on IBD may involve inhibition of eosinophils. The mechanism of anti‐inflammatory effect of DKT partially differs from that of 5‐ASA. Coapplication of DKT and conventional medicine may produce a positive synergy effect for IBD treatment. To the best of our knowledge, we demonstrated, for the first time, that (i) daikenchuto (DKT) suppressed colonic hypersensitivity and eosinophil infiltration associated with sodium dextran sulfate or trinitrobenzene sulfate‐induced colitis. (ii) The mechanism of anti‐inflammatory effect of DKT partially differs from that of 5‐aminosalicylic acid (5‐ASA). (iii) Coapplication of DKT and 5‐ASA may produce a positive synergy effect for IBD treatment.
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DOI：
10.1016/j.jcmgh.2017.12.005
发表时间：
2018-03
影响因子：
7.2
作者：
Kurahara LH;Hiraishi K;Hu Y;Koga K;Onitsuka M;Doi M;Aoyagi K;Takedatsu H;Kojima D;Fujihara Y;Jian Y;Inoue R
通讯作者：
Inoue R
影响因子：
6.3
作者：
Endo, Mari;Hori, Masatoshi;Ozaki, Hiroshi;Oikawa, Tetsuro;Hanawa, Toshihiko
通讯作者：
Hanawa, Toshihiko
影响因子：
24.5
作者：
Masterson JC;McNamee EN;Fillon SA;Hosford L;Harris R;Fernando SD;Jedlicka P;Iwamoto R;Jacobsen E;Protheroe C;Eltzschig HK;Colgan SP;Arita M;Lee JJ;Furuta GT
通讯作者：
Furuta GT
胃肠道嗜酸性粒细胞在免疫和疾病中的作用和调节。
DOI：
10.4049/jimmunol.1400413
发表时间：
2014-08-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Jung Y;Rothenberg ME
通讯作者：
Rothenberg ME
影响因子：
4.4
作者：
Forbes, E;Murase, T;Hogan, SP
通讯作者：
Hogan, SP