Daikenchuto, a traditional Japanese herbal medicine, ameliorates postoperative ileus by anti-inflammatory action through nicotinic acetylcholine receptors.
Daikenchuto, a traditional Japanese herbal medicine, ameliorates postoperative ileus by anti-inflammatory action through nicotinic acetylcholine receptors.
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DOI：
10.1007/s00535-013-0854-6
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发表时间：
2014-06
影响因子：
6.3
通讯作者：
Hanawa, Toshihiko
中科院分区：
文献类型：
--
作者：
Endo, Mari;Hori, Masatoshi;Ozaki, Hiroshi;Oikawa, Tetsuro;Hanawa, Toshihiko
关键词：
Daikenchuto（DKT）是一种促进胃肠动力的日本草药，用于腹部手术后出现术后肠梗阻（POI）和粘连性肠梗阻的患者。已经提出了通过DKT改善POI的几种机制;然而，DKT是否在POI中显示抗炎作用仍不清楚。在本研究中，我们研究了DKT在小鼠POI模型中的作用，并试图阐明详细的作用机制。将肠操作（IM）应用于小鼠的远端回肠。在IM之前和之后对动物经口给予DKT 4次。分析在体胃肠转运、白细胞浸润、细胞因子mRNA表达和胃肠动力。我们还研究了α 7 nAChR拮抗剂柠檬酸甲基甘草次酸（methylycaconitine citrate，MLA）对DKT介导的对POI的改善作用的影响，并研究了DKT对α 7 nAChR基因敲除小鼠炎症活动的影响。DKT治疗导致IM引起的延迟肠传输的恢复。DKT可显著抑制中性粒细胞和CD 68阳性巨噬细胞的浸润，抑制TNF-α和MCP-1 mRNA的表达。MLA显著降低DKT的抗炎作用，并部分抑制DKT对α 7 nAChR基因敲除小鼠巨噬细胞浸润的改善作用。总之，除了胃肠道促动力作用，DKT作为一种新的治疗药物POI的特点是其抗炎效力。DKT诱导的抗炎活性可能部分由α 7 nAChR的激活介导。
Daikenchuto (DKT), a gastrointestinal prokinetic Japanese herbal medicine, is prescribed for patients with postoperative ileus (POI) and adhesive bowel obstruction following abdominal surgery. Several mechanisms for the amelioration of POI by DKT have been suggested; however, it has remained unclear whether DKT shows anti-inflammatory effects in POI. In the present study, we investigated the effects of DKT in a mouse POI model and attempted to clarify the detailed mechanisms of action. Intestinal manipulation (IM) was applied to the distal ileum of mice. DKT was administered orally to the animals 4 times before and after IM. Gastrointestinal transit in vivo, leukocyte infiltration, cytokine mRNA expression and gastrointestinal motility were analyzed. We also investigated the effects of the α7nAChR antagonist methyllycaconitine citrate (MLA) on the DKT-mediated ameliorative action against POI, and we studied the effects of DKT on inflammatory activity in α7nAChR knockout mice. DKT treatment led to recovery of the delayed intestinal transit induced by IM. DKT significantly inhibited the infiltration of neutrophils and CD68-positive macrophages, and inhibited mRNA expressions of TNF-α and MCP-1. MLA significantly reduced the anti-inflammatory action of DKT, and the amelioration of macrophage infiltration by DKT was partially suppressed in α7nAChR knockout mice. In conclusion, in addition to the gastrointestinal prokinetic action, DKT serves as a novel therapeutic agent for POI characterized by its anti-inflammatory potency. The DKT-induced anti-inflammatory activity may be partly mediated by activation of α7nAChR.
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影响因子：
3.1
作者：
Jin, XL;Shibata, C;Sasaki, I
通讯作者：
Sasaki, I
影响因子：
2
作者：
Nagano, T;Itoh, H;Takeyama, M
通讯作者：
Takeyama, M
影响因子：
2.3
作者：
Kinoshita, Kazuya;Horiguchi, Kazuhide;Ozaki, Hiroshi
通讯作者：
Ozaki, Hiroshi
影响因子：
2.2
作者：
Kono, Toru;Koseki, Takashi;Kasai, Shinichi
通讯作者：
Kasai, Shinichi
DOI：
10.1254/jjp.86.32
发表时间：
2001-05-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
Satoh, K;Hashimoto, K;Nakamura, T
通讯作者：
Nakamura, T