Understanding and engineering RNA sequence specificity of PUF proteins.

Understanding and engineering RNA sequence specificity of PUF proteins.
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DOI:
10.1016/j.sbi.2008.12.009
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发表时间:
2009-02
影响因子:
6.8
通讯作者:
Hall, Traci M. Tanaka
Hall, Traci M. Tanaka
中科院分区:
生物学2区
文献类型:
--
作者:
Lu, Gang;Dolgner, Stephen J.;Hall, Traci M. Tanaka

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PUF 蛋白是 RNA 结合蛋白,以创始成员 PUMILIO 和 FBF 命名。这些蛋白质共同代表了一系列已知的 RNA 识别特性。 PUMILIO 是一种典型的 PUF 蛋白,其 RNA 序列特异性简单、优雅且可预测。 FBF 显示了 RNA 识别的差异,这代表了与原型的差异。在此,我们回顾了最近研究 PUF 蛋白序列特异性工程及其应用的研究,以及增进我们对该蛋白家族 RNA 识别天然多样性的理解的研究。
PUF proteins are RNA-binding proteins named for founding members PUMILIO and FBF. Together these proteins represent the range of known RNA recognition properties. PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable. FBF displays differences in RNA recognition that represent divergence from the prototype. Here we review recent studies that examine the engineering of sequence specificity of PUF proteins and its applications as well as studies that increase our understanding of the natural diversity of RNA recognition by this family of proteins.
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