Detailed Study of Cyanobacterial Microcystins Using High Performance Tandem Mass Spectrometry
Detailed Study of Cyanobacterial Microcystins Using High Performance Tandem Mass Spectrometry
复制标题
使用高性能串联质谱法详细研究蓝藻微囊藻毒素
DOI：
10.1007/s13361-014-0893-0
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发表时间：
2014
影响因子：
3.2
通讯作者：
Volmer DA
中科院分区：
文献类型：
--
作者：
Bortoli S;Volmer DA
微囊藻毒素（MC）是由富营养化水体中的蓝藻产生的一类具有毒性的环肽。MC变体的鉴定主要依赖于液相色谱（LC）结合碰撞诱导解离（CID）质谱法。偏离必需氨基酸补体是这些天然产物的共同特征，这使得CID分析更加困难并且并不总是成功的。在这里，CID和电子捕获解离（ECD）结合超高分辨率傅立叶变换离子回旋共振质谱法，研究蓝藻菌株分离自萨尔托格兰德水库在圣保罗州，巴西，没有事先LC分离。CID被证明是一种有效的解离技术，用于快速鉴定MC变体，即使是那些以前难以通过CID表征的变体。此外，ECD提供了更详细和补充的信息，这使我们能够精确地定位MC的金属结合位点的第一次。这些额外的信息对于环境化学家研究生态系统中MC的积累和产生将是重要的。
Microcystins (MC) are a large group of toxic cyclic peptides, produced by cyanobacteria in eutrophic water systems. Identification of MC variants mostly relies on liquid chromatography (LC) combined with collision-induced dissociation (CID) mass spectrometry. Deviations from the essential amino acid complement are a common feature of these natural products, which makes the CID analysis more difficult and not always successful. Here, both CID and electron capture dissociation (ECD) were applied in combination with ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry to study a cyanobacteria strain isolated from the Salto Grande Reservoir in Sao Paulo State, Brazil, without prior LC separation. CID was shown to be an effective dissociation technique for quickly identifying the MC variants, even those that have previously been difficult to characterize by CID. Moreover, ECD provided even more detailed and complementary information, which enabled us to precisely locate metal binding sites of MCs for the first time. This additional information will be important for environmental chemists to study MC accumulation and production in ecosystems.
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影响因子：
7.4
作者：
Diehnelt, CW;Dugan, NR;Budde, WL
通讯作者：
Budde, WL
影响因子：
1.3
作者：
Zubarev, RA;Haselmann, KF;Jensen, F
通讯作者：
Jensen, F
DOI：
10.1016/j.toxicon.2008.03.026
发表时间：
2008-06
期刊：
Toxicon : official journal of the International Society on Toxinology
影响因子：
--
作者：
Kazunori Saito;Y. Sei;S. Miki;K. Yamaguchi
通讯作者：
Kazunori Saito;Y. Sei;S. Miki;K. Yamaguchi
影响因子：
7.4
作者：
Zubarev, RA;Horn, DM;McLafferty, FW
通讯作者：
McLafferty, FW
影响因子：
7.4
作者：
Mosely, Jackie A.;Smith, Michael J. P.;Bristow, Anthony W. T.
通讯作者：
Bristow, Anthony W. T.