Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin.
Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin.
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DOI：
10.1083/jcb.61.2.557
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发表时间：
1974-05
影响因子：
7.8
通讯作者：
GOODENOU.DA
中科院分区：
文献类型：
--
作者：
GOODENOU.DA
间隙连接是多种组织中相邻细胞之间的特殊接触（1,2），被认为介导电紧张耦合(3)。这种结的特征是亚基的六边形晶格，可以在镧浸渍、负染色和冻裂制备中看到(1)。已经发表了一种从小鼠肝脏中分离形态完整的间隙连接的方法(4)，该方法利用了间隙连接在洗涤剂n-月桂醇肌氨酸中的独特不溶性。由于这种分离方法产生的材料数量少，对间隙结生物化学和结构的分析受到了限制。本通讯报告了一种大量分离肝细胞间隙连接的方法，产生适合于结构研究的毫克量的纯连接。此外，对间隙连接主要蛋白的化学性质也有了新的认识。
The gap junction is a specialized contact between adjacent cells in a wide variety of tissues (1, 2) which is thought to mediate electrotonic coupling (3). This junction is characterized by a hexagonal lattice of subunits which may be seen in lanthanum-impregnated, negative-stained, and freezefractured preparations (1). A method for isolation of morphologically intact gap junctions from mouse liver has been published (4), which takes advantage of the gap junction's unique insolubility in the detergent n-lauroyl sarcosine. Analysis of gap junction biochemistry and structure has been limited by the small amounts of material produced by this isolation method. This communication reports a method for bulk isolation of hepatocyte gap junctions, resulting in milligram quantities of pure junctions suitable for structural studies. In addition, new data are presented on the chemistry of the principal protein of the gap junction.
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影响因子：
7.8
作者：
GOODENOUGH, DA;REVEL, JP
通讯作者：
REVEL, JP
影响因子：
2.9
作者：
FAIRBANKS, G;STECK, TL;WALLACH, DFH
通讯作者：
WALLACH, DFH
影响因子：
56.9
作者：
DEWEY, MM;BARR, L
通讯作者：
BARR, L
影响因子：
7.8
作者：
GOODENOUGH, DA;STOECKENIUS, W
通讯作者：
STOECKENIUS, W