Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists.
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass-Spectrometry (MALDI-TOF MS) Based Microbial Identifications: Challenges and Scopes for Microbial Ecologists.
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DOI：
10.3389/fmicb.2016.01359
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发表时间：
2016
影响因子：
5.2
通讯作者：
Shouche YS
中科院分区：
文献类型：
--
作者：
Rahi P;Prakash O;Shouche YS
基于基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)的生物分型技术是一种高通量、快速鉴定微生物的新兴技术。由于其相对较高的准确性、全面的临床重要微生物数据库以及与其他微生物鉴定方法相比的低成本，MALDI-TOF MS已经开始取代临床诊断中普遍存在的现有方法。然而，MALDI-TOF MS在微生物生态学研究领域的适用性仍然有限，这主要是因为缺乏关于非临床微生物的数据。通过常规方法以及创新和高通量方法培养微生物多样性的密集研究活动大大增加了目前已知的微生物种类数量。这一重要的研究领域迫切需要快速可靠的方法(S)来表征和消除各种生态系统中的微生物。在我们看来，基于MALDI-TOF MS的表征似乎是最适合这类研究的技术。基于MALDI-TOF MS的识别方法的可靠性主要取决于参考数据库的精度和宽度，需要不断扩展和改进。在这篇综述中，我们提出了一种建立MALDI-TOF MS光谱数据库的通用策略，并倡导共享，并讨论了基于MALDI-TOF MS的高通量微生物鉴定在微生物生态学研究中的作用。
Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) based biotyping is an emerging technique for high-throughput and rapid microbial identification. Due to its relatively higher accuracy, comprehensive database of clinically important microorganisms and low-cost compared to other microbial identification methods, MALDI-TOF MS has started replacing existing practices prevalent in clinical diagnosis. However, applicability of MALDI-TOF MS in the area of microbial ecology research is still limited mainly due to the lack of data on non-clinical microorganisms. Intense research activities on cultivation of microbial diversity by conventional as well as by innovative and high-throughput methods has substantially increased the number of microbial species known today. This important area of research is in urgent need of rapid and reliable method(s) for characterization and de-replication of microorganisms from various ecosystems. MALDI-TOF MS based characterization, in our opinion, appears to be the most suitable technique for such studies. Reliability of MALDI-TOF MS based identification method depends mainly on accuracy and width of reference databases, which need continuous expansion and improvement. In this review, we propose a common strategy to generate MALDI-TOF MS spectral database and advocated its sharing, and also discuss the role of MALDI-TOF MS based high-throughput microbial identification in microbial ecology studies.
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影响因子：
3.7
作者：
Cibrián-Jaramillo A;Barona-Gómez F
通讯作者：
Barona-Gómez F
影响因子：
2.9
作者：
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Patel, Robin
DOI：
10.1016/j.ijantimicag.2014.04.020
发表时间：
2014-08-01
影响因子：
10.8
作者：
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通讯作者：
Raoult, Didier
影响因子：
3.4
作者：
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通讯作者：
Gerdts, Gunnar