Recurrent urinary tract infection and estrogen shape the taxonomic ecology and function of the postmenopausal urogenital microbiome.
Recurrent urinary tract infection and estrogen shape the taxonomic ecology and function of the postmenopausal urogenital microbiome.
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复发性尿路感染和雌激素塑造了绝经后泌尿生殖微生物组的分类生态学和功能。
DOI：
10.1016/j.xcrm.2022.100753
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发表时间：
2022-10-18
影响因子：
14.3
通讯作者：
Nisco, Nicole J. De
中科院分区：
文献类型：
--
作者：
Neugent, Michael L.;Kumar, Ashwani;Hulyalkar, Neha V.;Lutz, Kevin C.;Nguyen, Vivian H.;Fuentes, Jorge L.;Zhang, Cong;Nguyen, Amber;Sharon, Belle M.;Kuprasertkul, Amy;Arute, Amanda P.;Ebrahimzadeh, Tahmineh;Natesan, Nitya;Xing, Chao;Shulaev, Vladimir;Li, Qiwei;Zimmern, Philippe E.;Palmer, Kelli L.;Nisco, Nicole J. De
绝经后妇女严重受到复发性尿路感染（rUTI）的影响。泌尿生殖微生物组是泌尿环境的关键组成部分。然而，泌尿生殖系统微生物组的变化导致rUTI易感性尚不清楚。在这里，我们对绝经后妇女对照队列的尿液进行鸟枪元基因组学和高级培养，以确定与rUTI易感性相关的泌尿生殖微生物组组成和功能变化。我们确定了绝经后妇女rUTI易感性的候选分类生物标志物，以及绝经后接受雌激素激素治疗的妇女乳酸菌的富集。我们发现在没有尿路感染（UTI）病史的女性中，双歧杆菌和乳酸杆菌与尿雌激素之间存在强大的相关性。功能分析揭示了与rUTI相关的独特代谢和抗微生物药物耐药性基因（ARG）特征。重要的是，我们发现ARGs在有rUTI病史的女性的泌尿生殖微生物组中丰富，与目前的UTI状态无关。我们的数据表明，rUTI和雌激素塑造了绝经后妇女的泌尿生殖微生物群。复发性尿路感染改变绝经后泌尿生殖道微生物群的组成网络分析揭示了泌尿生殖道微生物群之间新的潜在相互作用泌尿雌激素与泌尿生殖道乳杆菌和双歧杆菌丰度相关泌尿生殖道微生物群中具有rUTI病史的抗菌素耐药性积累Neugent等人使用元基因组测序、高级尿液培养和代谢物分析来调查绝经后妇女的泌尿生殖道微生物群。发现与尿路感染复发易感性相关的临床重要关联。这些发现为未来发展绝经后妇女复发性尿路感染的新治疗和诊断策略提供了基础。
Postmenopausal women are severely affected by recurrent urinary tract infection (rUTI). The urogenital microbiome is a key component of the urinary environment. However, changes in the urogenital microbiome underlying rUTI susceptibility are unknown. Here, we perform shotgun metagenomics and advanced culture on urine from a controlled cohort of postmenopausal women to identify urogenital microbiome compositional and function changes linked to rUTI susceptibility. We identify candidate taxonomic biomarkers of rUTI susceptibility in postmenopausal women and an enrichment of lactobacilli in postmenopausal women taking estrogen hormone therapy. We find robust correlations between Bifidobacterium and Lactobacillus and urinary estrogens in women without urinary tract infection (UTI) history. Functional analyses reveal distinct metabolic and antimicrobial resistance gene (ARG) signatures associated with rUTI. Importantly, we find that ARGs are enriched in the urogenital microbiomes of women with rUTI history independent of current UTI status. Our data suggest that rUTI and estrogen shape the urogenital microbiome in postmenopausal women. Recurrent UTI changes the composition of the postmenopausal urogenital microbiome Network analysis reveals new potential interactions between urogenital microbial taxa Urinary estrogen correlates with urogenital Lactobacillus and Bifidobacterium abundance Antimicrobial resistance accumulates in urogenital microbiomes with rUTI history Neugent et al. use metagenomic sequencing, advanced urine culture, and metabolite profiling to survey the urogenital microbiome of postmenopausal women, finding clinically important associations relevant to recurrent UTI susceptibility. These findings provide a foundation for the future development of novel therapeutic and diagnostic strategies for recurrent UTI in postmenopausal women.
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DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Flores-Mireles AL;Walker JN;Caparon M;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
7.4
作者：
Fouts DE;Pieper R;Szpakowski S;Pohl H;Knoblach S;Suh MJ;Huang ST;Ljungberg I;Sprague BM;Lucas SK;Torralba M;Nelson KE;Groah SL
通讯作者：
Groah SL
影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
4.6
作者：
Ammitzbøll N;Bau BPJ;Bundgaard-Nielsen C;Villadsen AB;Jensen AM;Leutscher PDC;Glavind K;Hagstrøm S;Arenholt LTS;Sørensen S
通讯作者：
Sørensen S
影响因子：
3.6
作者：
Eriksson, Irene;Gustafson, Yngve;Olofsson, Birgitta
通讯作者：
Olofsson, Birgitta