Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung.
Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung.
复制标题
DOI：
10.1088/0957-4484/24/39/395101
复制
发表时间：
2013-10-04
期刊：
影响因子：
3.5
通讯作者：
Salem AK
中科院分区：
文献类型：
--
作者：
Worthington KL;Adamcakova-Dodd A;Wongrakpanich A;Mudunkotuwa IA;Mapuskar KA;Joshi VB;Allan Guymon C;Spitz DR;Grassian VH;Thorne PS;Salem AK
尽管铜纳米颗粒具有多种应用潜力，但在雾化递送后会引起非常强烈的炎症反应和细胞毒性。用生物相容性和抗菌壳聚糖等多糖包覆金属纳米粒子，有可能降低这种毒性。本研究采用一种新型的壳聚糖包覆铜纳米粒子的方法。利用x射线光电子能谱（XPS）对壳聚糖进行罗丹明标记，并对包覆后的壳聚糖颗粒进行共聚焦荧光成像，观察到壳聚糖颗粒大小和zeta电位的增加。进一步的物理和化学特性评价使用溶解和x射线衍射（XRD）研究。壳聚糖包被可显著降低铜纳米粒子在体外培养24小时和52小时后的毒性，以及24小时后DHE氧化测定的活性氧的生成。相反，暴露于壳聚糖包覆与未包覆的铜纳米颗粒的小鼠的支气管肺泡液中白细胞、总蛋白和细胞因子/趋化因子的数量测量显示，炎症反应增加，铜离子浓度也增加。这些结果表明，用黏附多糖（如壳聚糖）包裹金属纳米颗粒可以增加其在控制铜离子向细胞释放方面的潜力，但如果通过肺部给药，将导致更高的炎症反应。
Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy (XPS), rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles, observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction (XRD) studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 hours and the generation of reactive oxygen species as assayed by DHE oxidation after 24 hours in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the broncheoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
登录
查看更多内容
影响因子：
5.2
作者：
Chang, Ming-Hui;Liu, Hwai-Shen;Tai, Clifford Y.
通讯作者：
Tai, Clifford Y.
影响因子：
--
作者：
Osuna, Yolanda;Gregorio-Jauregui, Karla M.;Lopez, Raul G.
通讯作者：
Lopez, Raul G.
影响因子：
14
作者：
Kim, Sungwoo;Nishimoto, Satoru K.;Yang, Yunzhi
通讯作者：
Yang, Yunzhi
影响因子：
5.5
作者：
Ramu, Vasanthakumar Ganga;Bordoloi, Ankur;Cabrele, Chiara
通讯作者：
Cabrele, Chiara
影响因子：
5.4
作者：
No, HK;Park, NY;Meyers, SP
通讯作者：
Meyers, SP