The Mechanisms of Thiosulfate Toxicity against Saccharomyces cerevisiae.
The Mechanisms of Thiosulfate Toxicity against Saccharomyces cerevisiae.
复制标题
硫代硫酸盐对酿酒酵母的毒性机制
DOI：
10.3390/antiox10050646
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发表时间：
2021-04-22
期刊：
影响因子：
--
通讯作者：
Xun L
中科院分区：
文献类型：
--
作者：
Chen Z;Xia Y;Liu H;Liu H;Xun L
元素硫和亚硫酸盐已被用于抑制酵母的生长，但硫代硫酸盐尚未报道对酵母有毒。我们观察到硫代硫酸盐比亚硫酸盐更能抑制酿酒酵母在普通酵母培养基中的生长。在pH < 4时，硫代硫酸盐是元素硫和亚硫酸的来源，两者对酵母都是高毒性的。在pH 6时，硫代硫酸盐直接抑制酵母线粒体中的电子传递链，导致耗氧量、线粒体膜电位和细胞ATP降低。虽然硫代硫酸盐转化为亚硫酸盐和H2S的线粒体罗丹Rdl1，其毒性是不是由于H2S的rdl1缺失突变体，产生显着减少H2S比野生型更敏感的硫代硫酸盐。有证据表明硫代硫酸盐抑制酵母线粒体电子传递链中的细胞色素c氧化酶。因此，硫代硫酸盐是一种潜在的抗酵母菌剂。
Elemental sulfur and sulfite have been used to inhibit the growth of yeasts, but thiosulfate has not been reported to be toxic to yeasts. We observed that thiosulfate was more inhibitory than sulfite to Saccharomyces cerevisiae growing in a common yeast medium. At pH < 4, thiosulfate was a source of elemental sulfur and sulfurous acid, and both were highly toxic to the yeast. At pH 6, thiosulfate directly inhibited the electron transport chain in yeast mitochondria, leading to reductions in oxygen consumption, mitochondrial membrane potential and cellular ATP. Although thiosulfate was converted to sulfite and H2S by the mitochondrial rhodanese Rdl1, its toxicity was not due to H2S as the rdl1-deletion mutant that produced significantly less H2S was more sensitive to thiosulfate than the wild type. Evidence suggests that thiosulfate inhibits cytochrome c oxidase of the electron transport chain in yeast mitochondria. Thus, thiosulfate is a potential agent against yeasts.
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影响因子：
5.1
作者：
Houghton JL;Foustoukos DI;Flynn TM;Vetriani C;Bradley AS;Fike DA
通讯作者：
Fike DA
影响因子：
2.9
作者：
Melideo, Scott L.;Jackson, Michael R.;Jorns, Marilyn Schuman
通讯作者：
Jorns, Marilyn Schuman
影响因子：
11.4
作者：
NASH, R;TOKIWA, G;FUTCHER, AB
通讯作者：
FUTCHER, AB
影响因子：
2.8
作者：
Nardi, Tiziana;Corich, Viviana;Blondin, Bruno
通讯作者：
Blondin, Bruno
DOI：
10.1007/978-1-4614-3573-0_4
发表时间：
2012-01-01
期刊：
MITOCHONDRIAL OXIDATIVE PHOSPHORYLATION: NUCLEAR-ENCODED GENES, ENZYME REGULATION, AND PATHOPHYSIOLOGY
影响因子：
--
作者：
Ghezzi, Daniele;Zeviani, Massimo
通讯作者：
Zeviani, Massimo