OPN induces FoxM1 expression and localization through ERK 1/2, AKT, and p38 signaling pathway in HEC-1A cells.
OPN induces FoxM1 expression and localization through ERK 1/2, AKT, and p38 signaling pathway in HEC-1A cells.
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OPN 在 HEC-1A 细胞中通过 ERK 1/2、AKT 和 p38 信号通路诱导 FoxM1 表达和定位
DOI：
10.3390/ijms151223345
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发表时间：
2014-12-16
影响因子：
5.6
通讯作者：
Kong Y
中科院分区：
文献类型：
--
作者：
Xie Y;Li Y;Kong Y
哺乳动物胚胎着床是一个极其复杂的过程，需要子宫内膜具有容受性。为了建立这种容受性，月经周期中的连续增殖和分化是必要的。叉头框M1（FoxM1）被描述为肿瘤发生、促进和进展过程中的一个主要致癌转录因子。根据这些功能，我们认为FoxM1在胚胎着床中也应发挥重要作用。骨桥蛋白（OPN）是一种黏附分子，在生殖领域已被广泛研究。在本研究中，我们首先观察了FoxM1在人子宫内膜增殖期和分泌期以及小鼠着床前子宫中的表达和分布。然后我们观察了OPN和FoxM1之间的关系。我们的结果显示，FoxM1主要分布在腺上皮中。OPN以时间和浓度依赖的方式增加人子宫上皮细胞系HEC - 1A细胞中FoxM1的表达。OPN通过细胞外调节蛋白激酶（ERK 1/2）、蛋白激酶B（PKB，AKT）和p38丝裂原活化蛋白激酶（p38MAPK，p38）信号通路调节FoxM1，从而影响HEC - 1A细胞的增殖。抑制ERK 1/2、AKT和p38可抑制OPN诱导的FoxM1表达和定位。我们的数据表明，FoxM1可能受OPN调节，从而影响子宫内膜增殖以建立子宫内膜容受性。
Mammalian embryo implantation is an extremely complex process and requires endometrial receptivity. In order to establish this receptivity, sequential proliferation and differentiation during the menstrual cycle is necessary. Forkhead box M1 (FoxM1) is described as a major oncogenic transcription factor in tumor initiation, promotion and progression. According to these functions, we believe that FoxM1 should also play an essential role in embryo implantation. Osteopontin (OPN), an adhesion molecule, has been studied extensively in reproduction. In this study, we observed the expression and distribution of FoxM1 during the proliferative-phase and secretory-phase human endometrium and the pre-implantation mouse uterus firstly. Then we observed the relationship between OPN and FoxM1. Our results showed that FoxM1 was mainly distributed in glandular epithelium. OPN increased the expression of FoxM1 in the human uterine epithelial cell line HEC-1A cells in a time- and concentration-dependent manner. OPN regulates FoxM1 to influence HEC-1A cell proliferation through extracellular regulated protein kinases (ERK 1/2), protein kinase B (PKB, AKT), and the p38 mitogen activated protein kinases (p38MAPK, p38) signaling pathway. Inhibition of ERK 1/2, AKT and p38 suppressed OPN-induced FoxM1 expression and location. Our data indicate that FoxM1 might be regulated by OPN to influence endometrial proliferation to establish endometrial receptivity.
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影响因子：
3.6
作者：
Johnson, GA;Burghardt, RC;Bazer, FW
通讯作者：
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