Role of antibodies against outer-membrane proteins in murine resistance to infection with encapsulated Klebsiella pneumoniae.
Role of antibodies against outer-membrane proteins in murine resistance to infection with encapsulated Klebsiella pneumoniae.
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抗外膜蛋白抗体在小鼠抵抗封装肺炎克雷伯菌感染中的作用。
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Nomoto
中科院分区：
文献类型：
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作者：
B. Serushago;M. Mitsuyama;T. Handa;T. Koga;K. Nomoto
在对肺炎克雷伯菌包封毒力菌株及其荚膜多糖无毒突变株（CPS）的免疫评价中，两种菌株的灭活菌疫苗对小鼠的保护作用均为包封野生菌株的100倍LD50。每个菌株的抗血清在转移后对幼稚小鼠具有相同水平的保护；保护性抗突变血清对被包裹的细菌具有高度的调理能力。除了两种菌株共有的抗原成分外，野生菌株含有与突变株无关的抗原，因为抗野生血清的保护能力不受突变株预吸收的影响；抗突变血清的保护作用是由针对非荚膜抗原的抗体介导的，因为抗血清不含ELISA检测到的针对纯化CPS的抗体。作为非荚膜抗原的可能候选物，从突变菌株中提取的外膜蛋白（OMPs）进行了免疫原性检测。利用不含脂多糖的OMP对含蛋白部分进行免疫印迹和ELISA检测表明，肺炎克雷伯菌引起的免疫应答涉及多种蛋白。此外，用OMP或抗OMP血清免疫小鼠可以克服野生菌株的致死挑战。这些结果表明，肺炎克雷伯菌的OMPs可能是保护性抗原，并可能为开发非荚膜蛋白疫苗铺平道路。
In the assessment of immunity to the encapsulated virulent strain of Klebsiella pneumoniae and its avirulent mutant defective for capsular polysaccharide (CPS), killed bacterial vaccine of both strains could protect mice equally against challenge with 100 x LD50 of encapsulated wild strain. Antisera to each strain conferred the same level of protection on naive mice upon transfer; the protective anti-mutant serum was highly capable of opsonizing the encapsulated bacteria. In addition to the common antigenic components shared by both strains, the wild strain had antigen(s) unrelated to the mutant since the protective capacity of the anti-wild serum was not affected by preabsorption with the mutant strain; the protection conferred by the anti-mutant serum was mediated by antibodies against non-capsular antigens since the antiserum did not contain antibodies against purified CPS detectable by ELISA. As possible candidates among the non-capsular antigens, outer-membrane proteins (OMPs) extracted from the mutant strain were examined for their immunogenicity. Immunoblotting of the protein-containing fraction and ELISA using LPS-free OMP suggested that a number of proteins were involved in the immune response evoked by K. pneumoniae. Furthermore, mice immunized with OMP or anti-OMP serum could overcome a lethal challenge with the wild strain. These results indicated that OMPs of K. pneumoniae are implicated as the protective antigens and may pave the way for the development of non-capsular, proteinaceous vaccines.
奈瑟菌抗原 H.8 在播散性淋球菌和脑膜炎球菌感染患者中具有免疫原性。
DOI：
10.1093/infdis/151.4.650
发表时间：
1985
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Black,JR;Black,WJ;Cannon,JG
通讯作者：
Cannon,JG