Effect of reduced energy density of close-up diets on metabolites, lipolysis and gluconeogenesis in Holstein cows
Effect of reduced energy density of close-up diets on metabolites, lipolysis and gluconeogenesis in Holstein cows
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降低封闭日粮能量密度对荷斯坦奶牛代谢、脂肪分解和糖异生的影响
DOI：
10.5713/ajas.18.0624
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Cao Zhijun
中科院分区：
文献类型：
--
作者：
Huang Wenming;Wang Libin;Li Shengli;Cao Zhijun
目的研究降低能量密度的封闭式日粮对奶牛过渡期代谢产物、脂肪分解和脂肪生成的影响。方法将39头荷斯坦干奶牛随机分为3组，饲喂高能量密度日粮（HD，1.62 Mcal泌乳净能量[NEL]/kg干物质[DM]），中等能量密度饮食（MD，1.47 Mcal NEL/kg DM），或低能量密度饮食（LD，1.30 Mcal NEL/kg DM），它们被喂食相同的哺乳期饮食至28天的牛奶（DIM）。将所有奶牛圈养在自由畜栏中，自由采食。结果低能量饮食组大鼠在哺乳期血胰岛素浓度降低，非酯化脂肪酸（NEFA）浓度升高（p<0.05）。在泌乳期的前2周内，它们还增加了葡萄糖、胰岛素和胰高血糖素的浓度，降低了NEFA和β-羟基丁酸的浓度（p<0.05）。日粮能量密度对产后和产后血浆尿素氮浓度无显著影响（p>0.05）。过渡期内，饲料能量密度对脂肪组织中胰岛素受体、瘦素和过氧化物酶体增殖物激活受体-γ以及肝脏中磷酸烯醇式丙酮酸羧激酶、肉毒碱棕榈酰转移酶-1和过氧化物酶体增殖物激活受体-α的mRNA丰度没有影响（p>0.05）。与MD奶牛和LD奶牛相比，HD奶牛在3 DIM时具有更高的脂肪酶敏感性脂肪酶mRNA丰度（p = 0.001）。3 DIM时肝脏丙酮酸羧激酶的mRNA丰度往往会随着特写饲料能量密度的降低而增加（p = 0.08）。结论产后低能量饮食能有效控制脂肪组织动员，提高肝脏再生能力。
Objective An experiment was conducted to determine the effect of reduced energy density of close-up diets on metabolites, lipolysis and gluconeogenesis in cows during the transition period. Methods Thirty-nine Holstein dry cows were blocked and assigned randomly to three groups, fed a high energy density diet (HD, 1.62 Mcal of net energy for lactation [NEL]/kg dry matter [DM]), a medium energy density diet (MD, 1.47 Mcal NEL/kg DM), or a low energy density diet (LD, 1.30 Mcal NEL/kg DM) prepartum; they were fed the same lactation diet to 28 days in milk (DIM). All the cows were housed in a free-stall barn and fed ad libitum. Results The reduced energy density diets decreased the blood insulin concentration and increased nonesterified fatty acids (NEFA) concentration in the prepartum period (p<0.05). They also increased the concentrations of glucose, insulin and glucagon, and decreased the concentrations of NEFA and β-hydroxybutyrate during the first 2 weeks of lactation (p<0.05). The plasma urea nitrogen concentration of both prepartum and postpartum was not affected by dietary energy density (p>0.05). The dietary energy density had no effect on mRNA abundance of insulin receptors, leptin and peroxisome proliferator-activated receptor-γ in adipose tissue, and phosphoenolpyruvate carboxykinase, carnitine palmitoyltransferase-1 and peroxisome proliferator-activated receptor-α in liver during the transition period (p>0.05). The HD cows had higher mRNA abundance of hormone-sensitive lipase at 3 DIM compared with the MD cows and LD cows (p = 0.001). The mRNA abundance of hepatic pyruvate carboxykinase at 3 DIM tended to be increased by the reduced energy density of the close-up diets (p = 0.08). Conclusion The reduced energy density diet prepartum was effective in controlling adipose tissue mobilization and improving the capacity of hepatic gluconeogenesis postpartum.
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影响因子：
3.5
作者：
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