Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology
Profiling of the Causative Bacteria in Infected Lymphocysts after Lymphadenectomy for Gynecologic Cancer by Pyrosequencing the 16S Ribosomal RNA Gene Using Next-Generation Sequencing Technology
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使用下一代测序技术对 16S 核糖体 RNA 基因进行焦磷酸测序，对妇科癌症淋巴结切除术后感染的淋巴细胞中的致病细菌进行分析
DOI：
10.1155/2019/9326285
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发表时间：
2019
影响因子：
--
通讯作者：
Aoki Daisuke.
中科院分区：
文献类型：
--
作者：
Nogami Yuya;Banno Kouji;Adachi Masataka;Kunitomi Haruko;Kobayashi Yusuke;Tominaga Eiichiro;Aoki Daisuke.
背景妇科癌症的淋巴结切除术和盆腔放射治疗可产生淋巴囊肿，有时合并感染，导致盆腔炎。由于使用抗生素导致的假阴性结果和检测困难（包括厌氧菌），并不总是能发现真正的致病菌。使用16 S核糖体DNA通过下一代测序（NGS）分析细菌植物群可能会揭示细菌中真正的致病菌。这是第一次使用该技术对感染性淋巴囊肿的病原体进行研究。受试者为2015年7月至2016年9月在我院妇科癌症手术后发生感染性淋巴囊肿的患者。使用保存在-80 °C下的标本进行细菌植物群的NGS分析。对纯化的DNA样品进行两步PCR以获得序列文库。进行序列数据的处理，包括操作分类单位（OTU）定义、分类学分配和OTU BLAST检索。所有患者均签署了书面知情同意书，研究得到了机构研究伦理委员会的批准。6例患者接受穿刺引流。结果表明，大多数病例的病原体为单一病原体，包括路邓葡萄球菌、停乳链球菌、马链球菌、解糖肠球菌和大肠埃希菌。结论. NGS显示，淋巴囊肿感染的致病菌通常是单一菌株，如表面革兰氏阳性球菌或肠道细菌。应选择适当的抗生素以消除这些相应的细菌。
Background. Surgery for gynecologic cancer with lymphadenectomy and pelvic radiotherapy can produce lymphoceles that sometimes complicate with infection, resulting in abscesses. The true pathogenic bacteria of abscesses are not always found because of false‐negative results due to administered antibiotics and difficulty with detection, including for anaerobic bacteria. Analyzing bacteria flora by next‐generation sequencing (NGS) using 16S ribosomal DNA may reveal the true pathogenic bacteria in abscesses. This is the first report on causative pathogens for infectious lymphocele using this technology.Methods. The subjects were patients who developed infectious lymphocele after surgery for gynecologic cancer at our hospital from July 2015 to September 2016. NGS analyses of bacterial flora were performed using specimens preserved at ‐80°C. Two steps of PCR were performed for purified DNA samples to obtain sequence libraries. Processing of sequence data, including operational taxonomic unit (OTU) definition, taxonomy assignment, and an OTU BLAST search were performed. All patients gave written informed consent and the study was approved by the institutional research ethics committee.Results. Six patients underwent puncture and drainage. The result in most cases indicated a single causative pathogen, includingStaphylococcus lugdunensis, Streptococcus dysgalactiae, Streptococcus equinus, Enterococcus saccharolyticus,andEscherichia coli. Conclusions. NGS revealed that the causative bacteria in lymphocele infection are normally a single strain, such as a surface Gram‐positive coccus or enteric bacteria. Antibiotics should be chosen as appropriate for elimination of these respective bacteria.
影响因子：
2
作者：
Kawamura, Ichiro;Hirashima, Yasuyuki;Kurai, Hanako
通讯作者：
Kurai, Hanako
子宫内膜癌盆腔淋巴结清扫术后淋巴囊肿感染的危险因素分析及最佳引流时机
影响因子：
--
作者：
M. Pan;Y. Wang;Y. Yan;Q. Wang;S. Jiang;C. Han;X. H. Zhang;F. Xue
通讯作者：
F. Xue
DOI：
10.1097/igc.0000000000000353
发表时间：
2015-02-01
影响因子：
4.8
作者：
Hiramatsu, Kosuke;Kobayashi, Eiji;Kimura, Tadashi
通讯作者：
Kimura, Tadashi