Estrogen in cycling rats alters gene expression in the temporomandibular joint, trigeminal ganglia and trigeminal subnucleus caudalis/upper cervical cord junction.
Estrogen in cycling rats alters gene expression in the temporomandibular joint, trigeminal ganglia and trigeminal subnucleus caudalis/upper cervical cord junction.
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循环大鼠中的雌激素改变了颞下颌关节，三叉神经节和三叉核下caudalis/上颈椎索的基因表达。
DOI：
10.1002/jcp.22671
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发表时间：
2011-12
影响因子：
5.6
通讯作者：
Kramer, Phillip R.
中科院分区：
文献类型：
--
作者：
Puri, Jyoti;Bellinger, Larry L.;Kramer, Phillip R.
女性报告颞下颌关节（TMJ）疼痛比男性多，研究表明雌激素调节这种疼痛反应。我们在这项研究中的目标是确定受生理水平的17β-雌二醇调节的基因，这些基因可能在TMJ疼痛中起作用。为了完成这一目标，当血浆17β-雌二醇低或处于高发情前期水平时，在TMJ中注射盐水或完全弗氏佐剂。从治疗的大鼠中分离TMJ、三叉神经节和三叉神经尾侧亚核/上颈髓连接（Vc/C1-2）组织，并使用真实的时间PCR定量每个组织中的184个基因的表达。高、低雌激素组大鼠颞下颌关节组织、三叉神经节和Vc/C1-2区特异性转录物的数量有显著性差异。GABA A受体亚基α6（Gabra 6）和甘氨酸受体α2（Glra 2）是两个感兴趣的基因，因为它们在神经元活动中具有直接功能，并且在TMJ炎症的发情前期大鼠中观察到三叉神经节增加超过29倍。免疫组化结果显示，高雌激素组和低雌激素组大鼠神经元Gabrα6和Glrα2的表达均增加，而胶质细胞的表达无明显变化。雌激素受体α和β存在于三叉神经节的神经元中，因此17β-雌二醇可以改变Gabrα6和Glrα2的表达。雌激素受体α（ERα）在三叉神经节的卫星胶质细胞中表达，而ERβ则不表达。这些结果表明，与神经源性炎症或神经元兴奋性相关的基因因17β-雌二醇浓度的变化而改变。
Females report temporomandibular joint (TMJ) pain more than men and studies suggest estrogen modulates this pain response. Our goal in this study was to determine genes that are modulated by physiological levels of 17β-estradiol that could have a role in TMJ pain. To complete this goal, saline or complete Freund’s adjuvant was injected in the TMJ when plasma 17β-estradiol was low or when it was at a high proestrus level. TMJ, trigeminal ganglion and trigeminal subnucleus caudalis/upper cervical cord junction (Vc/C1–2) tissues were isolated from the treated rats and expression of 184 genes was quantitated in each tissue using real time PCR. Significant changes in the amount of specific transcripts were observed in the TMJ tissues, trigeminal ganglia and Vc/C1–2 region when comparing rats with high and low estrogen. GABA A receptor subunit α6 (Gabra6) and the glycine receptor α2 (Glra2) were two genes of interest because of their direct function in neuronal activity and a greater than 29 fold increase in the trigeminal ganglia was observed in proestrus rats with TMJ inflammation. Immunohistochemical studies showed that Gabrα6 and Glrα2 neuronal and not glial expression increased when comparing rats with high and low estrogen. Estrogen receptors α and β are present in neurons of the trigeminal ganglia, whereby 17β-estradiol can alter expression of Gabrα6 and Glrα2. Also, estrogen receptor α (ERα) but not ERβ was observed in satellite glial cells of the trigeminal ganglia. These results demonstrate that genes associated with neurogenic inflammation or neuronal excitability were altered by changes in the concentration of 17β-estradiol.
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影响因子：
2.9
作者：
BOHLHALTER, S;MOHLER, H;FRITSCHY, JM
通讯作者：
FRITSCHY, JM
影响因子：
5.3
作者：
Diogenes, Anibal;Patwardhan, Amol M.;Hargreaves, Kenneth M.
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Hargreaves, Kenneth M.
影响因子：
15.1
作者：
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通讯作者：
Hanani, Menachem
影响因子：
7.4
作者：
Chudler, EH;Anderson, LC;Byers, MR
通讯作者：
Byers, MR
影响因子：
1.9
作者：
Fischer, Luana;Arthuri, Mariana T.;Tambeli, Claudia Herrera
通讯作者：
Tambeli, Claudia Herrera