A novel one-step method to incorporate ss DNA into bilayer lipid membranes supported on an agar electrode

A novel one-step method to incorporate ss DNA into bilayer lipid membranes supported on an agar electrode
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一种将单链 DNA 整合到琼脂电极支持的双层脂膜中的新型一步法

DOI:
10.1016/j.elecom.2008.02.039
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发表时间:
2008-05
影响因子:
5.4
通讯作者:
--
中科院分区:
工程技术3区
文献类型:
--
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建立了一种一步法将ssDNA掺入琼脂糖电极上的双层类脂膜(BLM)中的方法,该方法对互补ssDNA有良好的响应。在BLM形成液中,ssDNA的5′端磷酸化末端与N,N-二乙基氨基苯(NDAB)的氨基形成了磷酰胺键。该方法操作简便,重现性好。傅里叶变换红外光谱(FTIR)分析表明,ss DNA已掺入到BLM中.
A one-step method has been developed for incorporating ss DNA into a bilayer lipid membrane (BLM) on agar electrode, which displayed good responses to complementary ss DNA. The 5′-terminal phosphated end of ss DNA formed a phosphormidate bond with the amino group of N,N-diethylamidobenzene (NDAB) in the BLM forming solution. The method is easy to perform and has good reproducibility. Fourier transform infrared spectroscopy (FTIR) showed that the ss DNA was incorporated into the BLM.
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