Ginsenoside ameliorated ventilator-induced lung injury in rats.
Ginsenoside ameliorated ventilator-induced lung injury in rats.
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DOI：
10.1186/s40560-020-00509-5
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发表时间：
2020-11-23
影响因子：
7.1
通讯作者：
Yeo HJ
中科院分区：
文献类型：
--
作者：
Cho WH;Kim YH;Heo HJ;Kim D;Kwak TW;Kim KH;Yeo HJ
人参皂苷具有抗氧化和抗炎功能。本研究旨在评估人参皂苷 Rb2 预处理对大鼠呼吸机所致肺损伤 (VILI) 的生物学效应。将大鼠分为四组，每组12只：对照组；低潮气量（TV），TV为6mL/kg，VILI，TV为20mL/kg，呼气末正压为5cmH2O，呼吸频率为每分钟60次呼吸，持续3小时，吸入氧分数为0.21；和人参皂苷，治疗与VILI组相同，但腹腔注射20mg/kg人参皂苷预处理。用显微镜观察形态以确认VILI模型。测量支气管肺泡灌洗液中的湿干重量比、蛋白质浓度和促炎细胞因子。对肺组织进行 RNA 测序以分析基因表达。高 TV 组织学诱发 VILI，伴有肺泡水肿和炎症细胞浸润。与 VILI 组相比，人参皂苷预处理显着降低了组织学肺损伤评分。 VILI 组的支气管肺泡灌洗液中的湿干重比、丙二醛和 TNF-α 显着较高，而人参皂苷预处理减轻了这些影响。在免疫组织化学检测中，人参皂苷预处理减弱了 VILI 诱导的 TNF-α 上调。与对照组相比，我们鉴定出 VILI 组中存在差异的 823 个基因。在 823 个基因中，只有 13 个基因（Arrdc2、Cygb、Exnef、Lcn2、Mroh7、Nsf、Rexo2、Srp9、Tead3、Ephb6、Mvd、Sytl4 和 Ube2l6）在人参皂苷组中恢复到对照水平。人参皂苷抑制 VILI 中的炎症和氧化应激反应。还需要对包括 LCN2 在内的 13 个基因进行进一步研究。
Ginsenosides have antioxidant and anti-inflammatory features. This study aimed to evaluate the biologic effects of ginsenoside Rb2 pretreatment on ventilator-induced lung injury (VILI) in rats. Rats were divided into four groups with 12 rats per group: control; low tidal volume (TV), TV of 6 mL/kg, VILI, TV of 20 mL/kg, positive end-expiratory pressure of 5 cm H2O, and respiratory rate of 60 breaths per minute for 3 h at an inspiratory oxygen fraction of 0.21; and ginsenosides, treated the same as the VILI group but with 20 mg/kg intraperitoneal ginsenoside pretreatment. Morphology was observed with a microscope to confirm the VILI model. Wet-to-dry weight ratios, protein concentrations, and pro-inflammatory cytokines in the bronchoalveolar lavage fluid were measured. RNA sequencing of the lung tissues was conducted to analyze gene expression. High TV histologically induced VILI with alveolar edema and infiltration of inflammatory cells. Ginsenosides pretreatment significantly reduced the histologic lung injury score compared to the VILI group. Wet-to-dry weight ratios, malondialdehyde, and TNF-α in bronchoalveolar lavage fluid were significantly higher in the VILI group and ginsenoside pretreatment mitigated these effects. In the immunohistochemistry assay, ginsenoside pretreatment attenuated the TNF-α upregulation induced by VILI. We identified 823 genes differentially presented in the VILI group compared to the control group. Of the 823 genes, only 13 genes (Arrdc2, Cygb, Exnef, Lcn2, Mroh7, Nsf, Rexo2, Srp9, Tead3, Ephb6, Mvd, Sytl4, and Ube2l6) recovered to control levels in the ginsenoside group. Ginsenosides inhibited the inflammatory and oxidative stress response in VILI. Further studies are required on the 13 genes, including LCN2.
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影响因子：
7.2
作者：
Oeckinghaus, Andrea;Ghosh, Sankar
通讯作者：
Ghosh, Sankar
影响因子：
4.6
作者：
Kwong, Lawrence N.;De Macedo, Mariana Petaccia;Gershenwald, Jeffrey E.
通讯作者：
Gershenwald, Jeffrey E.
影响因子：
2.9
作者：
Guo, Yan;Wu, Jie;Cai, Qiuyin
通讯作者：
Cai, Qiuyin
影响因子：
3.7
作者：
Lee JY;Linge HM;Ochani K;Lin K;Miller EJ
通讯作者：
Miller EJ
影响因子：
6.3
作者：
Kim JH;Yi YS;Kim MY;Cho JY
通讯作者：
Cho JY