Relationship between postprandial metabolomics and colon motility in children with constipation.
Relationship between postprandial metabolomics and colon motility in children with constipation.
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DOI：
10.1111/nmo.12086
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发表时间：
2013-05
影响因子：
3.5
通讯作者：
Goldstein AM
中科院分区：
文献类型：
--
作者：
Rodriguez L;Roberts LD;LaRosa J;Heinz N;Gerszten R;Nurko S;Goldstein AM
与结肠运动相关的代谢途径尚不清楚。为了确定治疗便秘的潜在代谢靶点，我们检查了一组便秘儿童在进餐前和进餐后的代谢谱，并确定了它与餐后结肠运动模式的关系。在这项前瞻性研究中，对接受结肠测压的便秘儿童进行了标准化进餐前后多个时间点的187种代谢物的液质联用测定。将餐后代谢物水平与基线进行比较，并与多项测压测量相关联，包括压力峰值的数量、频率和幅度以及运动指数(MI)。共纳入20名受试者，平均年龄(13.1±3.4)岁。餐后10分钟没有观察到显著的代谢物变化，而16种氨基酸和22种脂肪代谢物在餐后60分钟有显著的变化(P<；0.005)，包括甲基组胺、组胺和γ-氨基丁酸的减少。观察到正常和异常的餐后运动模式与特定代谢物的变化相关，包括甘油、肌氨酸、丙氨酸、天冬氨酸、胞嘧啶、胆碱、磷胆碱、甲状腺素和三碘甲状腺原氨酸。有趣的是，没有正常餐后曲线下面积增加(AUC)的受试者，犬尿酸和腺苷-同型半胱氨酸水平显著升高。这是第一个研究儿童餐后代谢变化并将特定代谢物的变化与结肠运动相关联的研究。这些结果提示了与运动有关的可能的代谢途径，并确定了治疗便秘的潜在靶点。
The metabolic pathways associated with colonic motility are unknown. To identify potential metabolic targets for treatment of constipation, we examined the metabolic profile before and after a meal challenge in a cohort of children with constipation and determined its relationship with postprandial colon motility patterns. In this prospective study, 187 metabolites were measured by liquid chromatography–mass spectrometry at multiple time points before and after a standardized meal in constipated children undergoing a colon manometry. Postprandial metabolite levels were compared with baseline and also correlated with multiple manometric measurements, including the number, frequency, and amplitude of pressure peaks as well as the motility index (MI). A total of 20 subjects were included (mean age 13.1 ± 3.4 years). No significant metabolite changes were observed at 10 min after the meal, whereas 16 amino acid and 22 lipid metabolites had significant (P < 0.005) postprandial changes, including decreases in methylhistamine, histamine, and GABA, by 60 min. Correlations were observed between normal and abnormal postprandial motility patterns and changes in specific metabolites, including glycerol, carnosine, alanine, asparagine, cytosine, choline, phosphocholine, thyroxine, and triiodothyronine. Interestingly, subjects without the normal postprandial increase in area under the curve (AUC), had markedly increased levels of kynurenic acid and adenosyl-homocysteine. This is the first study to examine postprandial metabolic changes in children and also to correlate changes in specific metabolites with colonic motility. The results suggest possible metabolic pathways associated with motility and identify potential targets for the treatment of constipation.
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影响因子：
9.9
作者：
Shaham, Oded;Wei, Ru;Wang, Thomas J.;Ricciardi, Catherine;Lewis, Gregory D.;Vasan, Ramachandran S.;Carr, Steven A.;Thadhani, Ravi;Gerszten, Robert E.;Mootha, Vamsi K.
通讯作者：
Mootha, Vamsi K.
影响因子：
24.5
作者：
Sander, LE;Lorentz, A;Bischoff, SC
通讯作者：
Bischoff, SC
DOI：
10.1097/00005176-199810000-00006
发表时间：
1998-10-01
影响因子：
2.9
作者：
Hamid, SA;Di Lorenzo, C;Hyman, PE
通讯作者：
Hyman, PE
DOI：
10.1152/ajpendo.00416.2002
发表时间：
2003-05-01
影响因子：
5.1
作者：
Tessari, P;Kiwanuka, E;Barazzoni, R
通讯作者：
Barazzoni, R
影响因子：
3.5
作者：
Kaszaki, J.;Palasthy, Z.;Boros, M.
通讯作者：
Boros, M.