Calcipotriene‐induced improvement in psoriasis is associated with reduced interleukin‐8 and increased interleukin‐10 levels within lesions
Calcipotriene‐induced improvement in psoriasis is associated with reduced interleukin‐8 and increased interleukin‐10 levels within lesions
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卡泊三烯诱导的银屑病改善与皮损内白细胞介素 8 水平降低和白细胞介素 10 水平升高相关
DOI：
10.1046/j.1365-2133.1998.02029.x
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发表时间：
1998
影响因子：
10.3
通讯作者：
Jee
中科院分区：
文献类型：
--
作者：
Sewon Kang;S. Yi;C. Griffiths;L. Fancher;T. Hamilton;Jee
卡泊三烯是 1,25-二羟基维生素 D3 的合成类似物，经证实可有效局部治疗牛皮癣。我们研究了卡泊三烯在银屑病中诱导的早期细胞和免疫学事件。 30 名中度斑块型银屑病患者被随机分配接受每天两次 0.005% 卡泊三烯软膏或匹配载体的应用，持续 6 周。在基线以及第 3 天和第 7 天对指定斑块进行皮肤活检 (6 mm)。在这些天以及第 2、4 和 6 周，以双盲方式进行完整的临床评估。与之前的研究一致，与接受赋形剂的患者相比，接受卡泊三烯治疗的患者在第 7 天的皮屑和红斑以及第 14 天的厚度方面观察到显着的临床改善 (P < 0.05)。然而，在第 3 天没有看到明显的改善。卡泊三烯治疗 7 天后，半定量评估的银屑病斑块中的免疫组织学标志物（CD1a、CD4、CD8、ICAM-1、VCAM-1、E-选择素、HLA-DR）均未发生显着变化。在卡泊三烯治疗组中，白细胞介素 (IL)-10 水平（pg/μg 蛋白质）从基线（0.030 ± 0.006；平均值 ± SEM）到第 3 天（0.047 ± 0.011）增加了 57%（与基线相比，P = 0.05；n = 10）并在第 7 天保持升高 (0.046±0.012)。然而，IL-8 水平（pg/μg 蛋白质）从基线 (0.13±0.06) 到第 3 天 (0.04±0.01) 下降了 70%，并在第 7 天保持较低水平 (0.03±0.02)（与基线相比，P< 0.05；n = 10）。 IL-8 和 IL-10 均不受媒介物处理的影响。卡泊三烯诱导银屑病临床改善之前，IL-10 水平增加，同时 IL-8 水平下降。这两种细胞因子水平的变化为免疫学变化作为卡泊三烯治疗银屑病作用机制的重要部分提供了进一步的证据。
Calcipotriene is a synthetic analogue of 1,25‐dihydroxyvitamin D3 established to be effective topically in the treatment of psoriasis. We investigated the early cellular and immunological events induced by calcipotriene in psoriasis. Thirty patients with moderate plaque‐type psoriasis were randomly assigned to receive twice daily applications of either calcipotriene ointment 0.005% or matching vehicle for 6 weeks. Skin biopsies (6 mm) were performed from designated plaques at baseline and days 3 and 7. On these days and at weeks 2, 4 and 6, complete clinical evaluations were made in a double‐blind fashion. Consistent with previous studies, significant clinical improvement (P < 0.05) in psoriasis was observed in patients receiving calcipotriene vs. those receiving vehicle by day 7 for scale and erythema, and by day 14 for thickness. No significant improvement, however, was seen on day 3. None of the immunohistological markers (CD1a, CD4, CD8, ICAM‐1, VCAM‐1, E‐selectin, HLA‐DR) semiquantitatively assessed in psoriatic plaques was significantly changed by calcipotriene treatment for 7 days. In the calcipotriene‐treated group, interleukin (IL)‐10 levels (pg/μg of protein) increased by 57% from baseline (0.030 ± 0.006; mean ± SEM) to day 3 (0.047 ± 0.011) (P = 0.05 vs. baseline; n = 10) and remained elevated at day 7 (0.046 ± 0.012). IL‐8 levels (pg/μg of protein), however, declined by 70% from baseline (0.13 ± 0.06) to day 3 (0.04 ± 0.01), and remained low at day 7 (0.03 ± 0.02) (P < 0.05 vs. baseline; n = 10). Both IL‐8 and IL‐10 were unaffected by vehicle treatment. Calcipotriene‐induced clinical improvement of psoriasis is preceded by an increase in IL‐10 and a concomitant decrease in IL‐8 levels. The changes in the level of these two cytokines provide further evidence for immunological changes as a significant part of the mechanism of action of calcipotriene in psoriasis.
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DOI：
10.1073/pnas.88.10.4538
发表时间：
1991-05-01
影响因子：
11.1
作者：
PARRONCHI, P;MACCHIA, D;ROMAGNANI, S
通讯作者：
ROMAGNANI, S
DOI：
10.1073/pnas.93.15.7861
发表时间：
1996-07-23
影响因子：
11.1
作者：
Cantorna, MT;Hayes, CE;DeLuca, HF
通讯作者：
DeLuca, HF
严重联合免疫缺陷小鼠和人类银屑病皮肤嵌合体。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Nickoloff,BJ;Kunkel,SL;Burdick,M;Strieter,RM
通讯作者：
Strieter,RM
影响因子：
56.9
作者：
KOCH, AE;POLVERINI, PJ;STRIETER, RM
通讯作者：
STRIETER, RM
银屑病角质形成细胞异常产生白细胞介素 8 和血小板反应蛋白 1，介导血管生成。
DOI：
--
发表时间：
1994
期刊：
The American journal of pathology
影响因子：
--
作者：
Nickoloff,BJ;Mitra,RS;Varani,J;Dixit,VM;Polverini,PJ
通讯作者：
Polverini,PJ