Plasmonic nanoprobes for SERS biosensing and bioimaging.

Plasmonic nanoprobes for SERS biosensing and bioimaging.
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DOI:
10.1002/jbio.200910015
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发表时间:
2010-01
影响因子:
2.8
通讯作者:
Scaffidi, Jonathan
Scaffidi, Jonathan
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Vo-Dinh, Tuan;Wang, Hsin-Neng;Scaffidi, Jonathan

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本文概述了我们实验室开发的用于生物传感和生物成像的等离子体纳米探针的开发和应用。我们描述了使用等离子体表面增强拉曼散射 (SERS) 基因探针通过 DNA 杂交来检测目标生物物种(HIV 基因、乳腺癌基因等)的疾病。对于分子成像,我们描述了一种高光谱表面增强拉曼成像 (HSERI) 系统,该系统将成像功能与 SERS 检测相结合,使用拉曼染料标记的银纳米颗粒来识别细胞系统中的细胞成分。介绍并讨论了使用“分子哨兵”纳米探针检测与乳腺癌相关的特定靶 DNA 序列,以及使用等离子体纳米传感器监测单细胞中的 pH 值。等离子体纳米传感器和纳米探针已被开发为环境监测、细胞生物传感、医疗诊断和高通量筛选的敏感和选择性工具。
This article provides an overview of the development and application of plasmonic nanoprobes developed in our laboratory for biosensing and bioimaging. We describe the use of plasmonics surface-enhanced Raman scattering (SERS) gene probes for the detection of diseases using DNA hybridization to target biospecies (HIV gene, breast cancer genes etc.). For molecular imaging, we describe a hyperspectral surface-enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components using Raman dye-labeled silver nanoparticles in cellular systems The detection of specific target DNA sequences associated with breast cancer using “molecular sentinel” nanoprobes and the use of a plasmonic nanosensor to monitor pH in single cells are presented and discussed. Plasmonic nanosensors and nanoprobes have been developed as sensitive and selective tolls for environmental monitoring, cellular biosensing, medical diagnostics and high throughput screnning.
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