Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machinery.
Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machinery.
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DOI：
10.7554/elife.79480
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发表时间：
2022-09-05
期刊：
影响因子：
7.7
通讯作者：
Mizuuchi, Kiyoshi
中科院分区：
文献类型：
--
作者：
Volante, Andrea;Alonso, Juan Carlos;Mizuuchi, Kiyoshi
三组分ParABS分配系统确保许多细菌染色体和低拷贝数质粒的稳定遗传。帕拉通过其ATP依赖性非特异性DNA结合活性定位于类核，而着丝粒样parS-DNA和ParB形成分配复合物，其激活ParA-ATP酶以驱动系统动力学。基本的parS序列安排不同的ParABS系统，反映了其分区复合物的建筑多样性。在这里，我们专注于pSM 19035质粒分配系统，使用的ParBpSM的带状螺旋螺旋（RHH）家庭。我们表明，parSpSM与四个或更多个连续的ParBpSM结合序列重复是必需的组装一个稳定的ParApSM-ParBpSM复合物，并有效地激活ParApSM-ATP酶，刺激复杂的拆卸。parSpSM序列阵列的邻接性的破坏使ParApSM-ParBpSM复合物不稳定并阻止有效的ATP酶活化。我们的研究结果揭示了pSM 19035分区复合物的独特结构以及它如何与核苷酸结合的ParApSM-ATP相互作用。
Three-component ParABS partition systems ensure stable inheritance of many bacterial chromosomes and low-copy-number plasmids. ParA localizes to the nucleoid through its ATP-dependent nonspecific DNA-binding activity, whereas centromere-like parS-DNA and ParB form partition complexes that activate ParA-ATPase to drive the system dynamics. The essential parS sequence arrangements vary among ParABS systems, reflecting the architectural diversity of their partition complexes. Here, we focus on the pSM19035 plasmid partition system that uses a ParBpSM of the ribbon-helix-helix (RHH) family. We show that parSpSM with four or more contiguous ParBpSM-binding sequence repeats is required to assemble a stable ParApSM-ParBpSM complex and efficiently activate the ParApSM-ATPase, stimulating complex disassembly. Disruption of the contiguity of the parSpSM sequence array destabilizes the ParApSM-ParBpSM complex and prevents efficient ATPase activation. Our findings reveal the unique architecture of the pSM19035 partition complex and how it interacts with nucleoid-bound ParApSM-ATP.
影响因子：
3.7
作者：
Lioy VS;Volante A;Soberón NE;Lurz R;Ayora S;Alonso JC
通讯作者：
Alonso JC
影响因子：
16
作者：
Han YW;Mizuuchi K
通讯作者：
Mizuuchi K
影响因子：
14.9
作者：
Pratto F;Cicek A;Weihofen WA;Lurz R;Saenger W;Alonso JC
通讯作者：
Alonso JC