Gamma-aminobutyrate-like immunoreactivity in the thalamus of the cat
Gamma-aminobutyrate-like immunoreactivity in the thalamus of the cat
复制标题
猫丘脑中的γ-氨基丁酸样免疫反应性
DOI：
10.1016/0306-4522(87)90037-6
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
J. Storm
中科院分区：
文献类型：
--
作者：
E. Rinvik;O. Ottersen;J. Storm
用提纯的抗γ-氨基丁酸抗血清孵育猫丘脑连续切片，用蒸馏戊二醛将其与牛血清白蛋白偶联。这种血清具有广泛的特征，并且似乎与戊二醛处理过的脑组织中固定的伽马氨基丁酸发生选择性反应。相邻的切片被硫素染色，为正确评价免疫标记模式提供了宝贵的指导。在神经束中，免疫染色的强度在丘脑核之间甚至在核的不同细分之间差异很大。在外侧膝状体旁核、室周核、中央内侧核、孤束核、斜方核、外侧缰核、内侧中央核、束旁核、束下核、内侧膝状体背侧、腹侧部、内侧膝状体背侧、后复合体、膝状体上核、枕部和部分后外侧后核中，神经纤维染色尤其浓染。枕骨/后外侧复合体显示出一种分化特别好的染色模式，与Updyke的[Updyke(1983)J.Comp]非常接近。神经这一区域的分割。在丘脑的几个核或亚区，尤其是那些投射到非初级感觉皮质区的中继核，免疫染色的神经纤维的特征是许多斑点环绕着一个未染色的轮廓。除极少数例外，所有丘脑核团都显示免疫反应阳性的神经细胞体。有几个例子表明，这类细胞的数量与神经纤维束的染色强度不匹配。因此，脑室周围核、束旁核、限束核和中间中心虽然有丰富的神经束染色，但几乎没有免疫染色的核周。粗略估计了伽玛氨基丁酸标记的神经元在丘脑所有大核和一些小核及其分支中的大小和比例。在丘脑的一些核，特别是网状核、前背侧核、背外侧核、中央外侧核、腹后核和外侧膝状体背侧核中，免疫反应阳性神经元的数量在胞体大小方面具有明显的异质性。特别强调在这方面似乎存在巨大的物种差异。
Serial sections of the cat's thalamus were incubated with a purified antiserum raised against gamma-aminobutyric acid conjugated to bovine serum albumin by distilled glutaraldehyde. This serum has been extensively characterized and appears to react selectively with fixed gamma-aminobutyric acid in brain tissue treated with glutaraldehyde. Adjoining sections were stained with thionin and served as invaluable guides for a correct evaluation of the immunolabelling pattern.In the neuropil the intensity of the immunostaining varies considerably between thalamic nuclei and even between nuclear subdivisions. The neuropil staining appears particularly dense in the nuclei parataenialis, periventricularis, centralis medialis, reuniens, rhomboideus, habenularis lateralis, centrum medianum, parafascicularis, subparafascicularis, submedius, dorsal and ventral parts of the lateral geniculate body, the dorsal part of the medial geniculate body, the posterior complex, suprageniculate nucleus, pulvinar and parts of the lateral posterior nucleus. The pulvinar/lateralis posterior complex shows a particularly well-differentiated staining pattern which closely matches Updyke's [Updyke (1983)J. comp. Neurol.219143–181] parcellation of this region. In several thalamic nuclei or subareas—and notably in those relay nuclei which are known to project upon non-primary sensory cortical areas—the immunostained neuropil is characterized by many puncta encircling an unstained profile.With few exceptions all thalamic nuclei displayed immunoreactive nerve cell bodies. Several examples were found of a mismatch between the number of such cells and the staining intensity of the neuropil. Thus the nuclei periventricularis, parafascicularis, subparafascicularis, parataenialis, limitans and centrum medianum although being very rich in neuropil staining have practically no immunostained perikarya.Rough estimates were made of the size and the proportion of gamma-aminobutyric acid labelled neurons in all major—and some minor—thalamic nuclei and their subdivisions. In some thalamic nuclei, notably the nuclei reticularis, anterior dorsalis, lateralis dorsalis, centralis lateralis, ventralis posterior and the dorsal lateral geniculate body, the population of immunoreactive neurons is distinctly heterogeneous with regard to soma size.The findings are discussed with regard to previous immunocytochemical studies of the distribution of gamma-aminobutyric acid and its synthesizing enzyme in the thalamus. Particular emphasis is put on the great species differences which appear to exist in this respect.
DOI：
--
发表时间：
1981
期刊：
International review of physiology
影响因子：
--
作者：
Jones Eg
通讯作者：
Jones Eg
丘脑腹基底复合体功能成分在猴体感皮层上的投射模式。
影响因子：
2.5
作者：
Jones,EG;Friedman,DP
通讯作者：
Friedman,DP
影响因子：
2.5
作者：
CRAIG, AD;BURTON, H
通讯作者：
BURTON, H