Comparative analysis of host-cell signalling mechanisms activated in response to infection with Rickettsia conorii and Rickettsia typhi.
Comparative analysis of host-cell signalling mechanisms activated in response to infection with Rickettsia conorii and Rickettsia typhi.
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康氏立克次体和伤寒立克次体感染激活的宿主细胞信号传导机制的比较分析。
DOI：
10.1099/jmm.0.47050-0
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发表时间：
2007
影响因子：
3
通讯作者：
Sahni,SanjeevK
中科院分区：
文献类型：
--
作者：
Rydkina,Elena;Sahni,Abha;Silverman,DavidJ;Sahni,SanjeevK
The Gram-negative intracellular bacteriaRickettsia conoriiandRickettsia typhiare the aetiological agents of Mediterranean spotted fever and endemic typhus, respectively, in humans. Infection of endothelial cells (ECs) lining vessel walls, and the resultant vascular inflammation and haemostatic alterations are salient pathogenetic features of both of these rickettsial diseases. An important consideration, however, is that dramatic differences in the intracellular motility and accumulation patterns for spotted fever versus typhus group rickettsiae have been documented, suggesting the possibility of unique and potentially different interactions with host cells. This study characterized and comparedR. conorii- andR. typhi-mediated effects on cultured human ECs. The DNA-binding activity of nuclear transcription factor-κB (NF-κB) and the phosphorylation status of stress-activated p38 kinase were determined as indicators of NF-κB and p38 activation.R. conoriiinfection resulted in a biphasic activation of NF-κB, with an early increase in DNA-binding activity at 3 h, followed by a later peak at 24 h. The activated NF-κB species were composed mainly of RelA p65–p50 heterodimers and p50 homodimers.R. typhiinfection of ECs resulted in only early activation of NF-κB at 3 h, composed primarily of p65–p50 heterodimers. WhilstR. conoriiinfection induced increased phosphorylation of p38 kinase (threefold mean induction) with the maximal response at 3 h, a considerably less-intense response peaking at about 6 h post-infection was found withR. typhi. Furthermore, mRNA expression of the chemokines interleukin (IL)-8 and monocyte chemoattractant protein-1 in ECs infected with eitherRickettsiaspecies was higher than the corresponding controls, but there were distinct differences in the secretion patterns for IL-8, suggesting the possibility of involvement of post-transcriptional control mechanisms or differences in the release from intracellular storage sites. Thus, the intensity and kinetics of host-cell responses triggered by spotted fever and typhus species exhibit distinct variations that could subsequently lead to differences in the extent of endothelial activation and inflammation and serve as important determinants of pathogenesis.
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E-选择素依赖性中性粒细胞粘附于立克次体感染的内皮细胞。
影响因子：
20.3
作者：
Sporn,LA;Lawrence,SO;Silverman,DJ;Marder,VJ
通讯作者：
Marder,VJ
“无细胞”系统中立克次体与内皮核因子-κB 的相互作用
影响因子：
5.2
作者：
S. Sahni;E. Rydkina;S. Joshi;L. Sporn;D. Silverman
通讯作者：
D. Silverman
康氏立克次体感染的内皮细胞中冯维勒布兰德因子的释放以及血栓调节蛋白和组织因子的表达
影响因子：
3.1
作者：
N. Teysseire;D. Arnoux;F. George;J. Sampol;D. Raoult
通讯作者：
D. Raoult
立克次体人内皮细胞胞浆提取物中核因子 κB 的蛋白酶体依赖性激活
影响因子：
3.1
作者：
S. Sahni;D. V. Van Antwerp;M. Eremeeva;D. Silverman;V. Marder;L. Sporn
通讯作者：
L. Sporn
基于立克次体肌动蛋白的运动
DOI：
--
发表时间：
2003
期刊：
影响因子：
--
作者：
R. Heinzen
通讯作者：
R. Heinzen