Fei-Liu-Ping ointment inhibits lung cancer growth and invasion by suppressing tumor inflammatory microenvironment.
Fei-Liu-Ping ointment inhibits lung cancer growth and invasion by suppressing tumor inflammatory microenvironment.
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肺瘤平膏通过抑制肿瘤炎症微环境抑制肺癌生长和侵袭
DOI：
10.1186/1472-6882-14-153
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发表时间：
2014-05-12
影响因子：
--
通讯作者：
Hua B
中科院分区：
文献类型：
--
作者：
Li W;Chen C;Saud SM;Geng L;Zhang G;Liu R;Hua B
背景肺癌是世界范围内癌症相关死亡的主要原因之一。常规化疗和放疗是肺癌的主要治疗方法，联合治疗的应用日益普及。治疗的频率和持续时间以及通过靶向癌症生物学的多个方面来管理肺癌通常受到对患者的毒性的限制。有许多天然存在的抗癌剂具有高度的功效和低毒性，为肺癌的治疗提供了可行且安全的方法。中药传统上用于抗癌治疗的草药提供了很大的潜力，以提高常规治疗的疗效。在这项研究中，我们评估了肺瘤平（FLP）软膏在治疗肺癌的协同作用;一个已知的抗癌中药为基础的formuls.MethodsIn这项研究中，A549人肺癌细胞系和刘易斯肺癌异种移植小鼠模型。此外，我们利用anin vitroco-culture系统来模拟肿瘤微环境，以评估的分子机制FLP treatment.ResultsFLP治疗显着抑制肿瘤生长的刘易斯肺异种移植物的40%，相比，环磷酰胺（CTX）的62.02%。FLP联合CTX对肿瘤生长的抑制率为83.23%。经过评估，我们发现FLP治疗降低了血清促炎细胞因子IL-6、TNF-α和IL-1β的浓度。此外，我们还发现了E-钙粘蛋白表达的改善和N-钙粘蛋白和MMP 9的抑制。我们在体外将A549细胞与巨噬细胞共培养时也发现了类似的结果。FLP抑制A549细胞的生长、侵袭和转移，其机制可能与调节NF-κB，改变E-cadherin、N-cadherin、MMP 2和MMP 9的表达有关。结论FLP具有抗炎作用，可能与其抗肿瘤作用有关。FLP治疗可能是一种有前途的治疗炎症相关的肺癌治疗单独，或与常规治疗相结合，并可能防止肺癌转移。
BackgroundLung cancer is one of the leading causes of cancer-related mortality worldwide. Conventional chemotherapy and radiotherapy are the primary therapeutic methods for lung cancer with the use of combination therapies gaining popularity. The frequency and duration of treatment, as well as, managing lung cancer by targeting multiple aspects of cancer biology is often limited by toxicity to the patient. There are many naturally occurring anticancer agents that have a high degree of efficacy and low toxicity, offering a viable and safe approach for the treatment of lung cancer. The herbs traditionally used in Chinese medicine for anticancer treatment offer great potential to enhance the efficacy of conventional therapy. In this study, we evaluated the synergistic effects of Fei-Liu-Ping (FLP) ointment in treating lung cancer; a known anticancer Chinese herbal based formula.MethodsIn this study, A549 human lung carcinoma cell line and Lewis lung carcinoma xenograft mouse model were used. In addition, we utilized anin vitroco-culture system to simulate the tumor microenvironment in order to evaluate the molecular mechanisms of FLP treatment.ResultsFLP treatment significantly inhibited tumor growth in the Lewis lung xenograft by 40 percent, compared to that of cyclophosphamide (CTX) of 62.02 percent. Moreover, combining FLP and CTX inhibited tumor growth by 83.23 percent. Upon evaluation, we found that FLP treatment reduced the concentration of serum pro-inflammatory cytokines IL-6, TNF-α, and IL-1β. In addition, we also found an improvement in E-cadherin expression and inhibition of N-cadherin and MMP9. We found similar findingsin vitrowhen we co-cultured A549 cells with macrophages. FLP treatment inhibited A549 cell growth, invasion and metastasis, in part, through the regulation of NF-κB and altering the expression of E-cadherin, N-cadherin, MMP2 and MMP9.ConclusionsFLP exerts anti-inflammatory properties in the tumor microenvironment, which may contribute to its anticancer effects. FLP treatment may be a promising therapy for inflammation associated lung cancer treatment alone, or in combination with conventional therapies and may prevent lung cancer metastasis.
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影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.0705316104
发表时间：
2007-11-20
影响因子：
11.1
作者：
Stathopoulos, Georgios T.;Sherrill, Taylor P.;Blackwell, Timothy S.
通讯作者：
Blackwell, Timothy S.
影响因子：
5.3
作者：
Tang, JM;He, QY;Chang, XJ
通讯作者：
Chang, XJ
影响因子：
4.4
作者：
Hagemann, T;Wilson, J;Balkwill, FR
通讯作者：
Balkwill, FR
影响因子：
5.2
作者：
Stathopoulos, Georgios T.;Sherrill, Taylor P.;Fingleton, Barbara
通讯作者：
Fingleton, Barbara