Multilevel control of extracellular sucrose metabolism in Streptococcus salivarius by sucrose.
Multilevel control of extracellular sucrose metabolism in Streptococcus salivarius by sucrose.
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蔗糖对唾液链球菌细胞外蔗糖代谢的多级控制。
DOI：
10.1099/00221287-137-1-5
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Bleiweis,AS
中科院分区：
文献类型：
--
作者：
Townsend-Lawman,P;Bleiweis,AS
建立标准化实验条件，检验蔗糖对唾液链球菌代谢的调控作用。 S 的新鲜分离物。使用唾液。在蔗糖上生长的细胞的胞外葡聚糖酶活性比在葡萄糖、果糖或半乳糖上生长的细胞高10倍。向半乳糖生长的细胞中添加蔗糖后，该活性在不到 5 分钟内增加，这种现象不受分别抑制转录和翻译的利福平和氯霉素的影响。当细胞在蔗糖上生长时，细胞外果聚糖酶活性比在其他糖上生长时高 2 倍。这种增加也在 5 分钟内发生，但被转录和翻译抑制剂减弱。细胞外葡萄糖基转移酶 (GTF) 活性的产生需要从头合成，在添加蔗糖后，GTF 活性与细胞表面结合。相反，细胞相关的果糖基转移酶（FTF）活性似乎需要遗传诱导才能产生和细胞表面关联，但需要蔗糖才能从表面框架释放。这组复杂酶的控制机制的多功能性使得它们的表达和功能能够在这些蛋白质生命史的几个广泛分离的阶段受到调节。
Standardized experimental conditions were established to test the role of sucrose in the regulation and control of its metabolism inStreptococcus salivarius. A fresh isolate ofS. salivariuswas used. The extracellular dextranase activity of cells grown on sucrose was 10-fold higher than that of cells grown on glucose, fructose or galactose. This activity increased in less than 5 min following the addition of sucrose to galactose-grown cells, a phenomenon which was affected by neither rifampicin nor chloramphenicol which inhibit transcription and translation, respectively. Extracellular fructanase activity was 2-fold higher when cells were grown on sucrose than when they were grown on the other sugars. This increase also occurred within 5 min, but was diminished by transcriptional and translational inhibitors.De novosynthesis was required for the production of extracellular glucosyltransferase (GTF) activity which, upon the addition of sucrose, became associated with the cell surface. Conversely, cell-associated fructosyltransferase (FTF) activity appeared to require genetic induction for its production and cell-surface association, but required sucrose for its release from the surface framework. Versatility in the control mechanisms of this complex set of enzymes allows their expression and function to be regulated at several widely separated stages in the life histories of these proteins.
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影响因子：
3.1
作者：
V. Schröder;S. Michalek;F. Macrina
通讯作者：
V. Schröder;S. Michalek;F. Macrina
影响因子：
3.5
作者：
Shunsaku Ueda;T. Shiroza;Howard K. Kuramitsu
通讯作者：
Shunsaku Ueda;T. Shiroza;Howard K. Kuramitsu
从唾液链球菌中分离编码蔗糖酶基因的 DNA 并对大肠杆菌中表达的酶进行部分表征
影响因子：
3.2
作者：
C. Houck;J. Pear;R. Elliott;J. Perchorowicz
通讯作者：
J. Perchorowicz
辛基β-D-吡喃葡萄糖苷抑制唾液链球菌中细胞相关果糖基转移酶的表达。
DOI：
--
发表时间：
1985
期刊：
Journal of General Microbiology
影响因子：
--
作者：
N. A. Jacques
通讯作者：
N. A. Jacques
细胞结合葡聚糖蔗糖酶与变形链球菌凝集的关系
影响因子：
3.1
作者：
M. M. Mccabe;E. Smith
通讯作者：
E. Smith