Human breast milk-derived exosomes attenuate cell death in intestinal epithelial cells.
Human breast milk-derived exosomes attenuate cell death in intestinal epithelial cells.
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DOI：
10.1177/1753425918785715
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发表时间：
2018-07
期刊：
影响因子：
3.2
通讯作者：
Sims B
中科院分区：
文献类型：
--
作者：
Martin C;Patel M;Williams S;Arora H;Brawner K;Sims B
母乳已被证明可以降低坏死性小肠结肠炎 (NEC) 的发病率。母乳含有多种成分（免疫球蛋白、蛋白质、脂肪以及最近引起关注的外泌体），但提供针对 NEC 保护的具体成分尚不清楚。外泌体是富含蛋白质、脂质和 microRNA 的小纳米囊泡。在这里，我们假设人母乳来源的外泌体可以保护肠上皮细胞（IEC）免于细胞死亡。收集人母乳，使用超速离心分离，并使用 NanoSight 跟踪分析进行定量。将纯化的外泌体添加到经过不同浓度的 H2O2 处理的 IEC 中。然后将细胞与人母乳来源的外泌体一起孵育过夜，并评估细胞活力。蛋白质印迹分析显示纯化样品中存在网格蛋白和 CD81。使用 H2O2 的氧化应激导致细胞活力下降 50%，而人母乳来源的外泌体对 IEC 具有保护作用。在 H2O2 存在的情况下，外泌体具有统计学上显着的保护作用。人类母乳来源的外泌体的保护作用并未因放线菌酮而减弱。因此，人母乳来源的外泌体可以保护 IEC 免受氧化应激，但其机制仍不清楚。对于患有肠道损伤的儿童来说，源自人母乳的外泌体是一种有吸引力的治疗概念。
Human breast milk has been shown to reduce the incidence of necrotizing enterocolitis (NEC). Breast milk has many components (immunoglobulins, proteins, fat, and, of recent interest, exosomes), but the specific component that affords protection against NEC is not known. Exosomes are small-nanometer vesicles that are rich in protein, lipid, and microRNA. Here, we hypothesized that human breast milk-derived exosomes can protect intestinal epithelial cells (IECs) from cell death. Human breast milk was collected, separated using ultracentrifugation, and quantified using NanoSight tracking analysis. Purified exosomes were added to IECs that had been treated with varying concentrations of H2O2. Cells were then incubated overnight with the human breast milk-derived exosomes and assessed for cell viability. Western blot analysis showed that both clathrin and CD81 were present in the purified sample. Oxidative stress using H2O2 caused a 50% decrease in cell viability and human breast milk-derived exosomes had a protective effect in IECs. In the presence of H2O2, exosomes had a statistically significant protective effect. The protection seen by human breast milk-derived exosomes was not attenuated by cycloheximide. Thus, human breast milk-derived exosomes allow IECs to be protected from oxidative stress, but the mechanism is still not clear. Exosomes derived from human breast milk are an attractive treatment concept for children with intestinal injury.
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影响因子：
8
作者：
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通讯作者：
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影响因子：
2.9
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影响因子：
4.5
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Lim B
影响因子：
8
作者：
Sims B;Farrow AL;Williams SD;Bansal A;Krendelchtchikov A;Gu L;Matthews QL
通讯作者：
Matthews QL
影响因子：
30.3
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通讯作者：
Bier E