Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food.
Natural Products Self-Assembled Nanozyme for Cascade Detection of Glucose and Bacterial Viability in Food.
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天然产物自组装纳米酶用于食品中葡萄糖和细菌活力的级联检测
DOI：
10.3390/foods10112596
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发表时间：
2021-10-27
期刊：
影响因子：
--
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Zhang Q;Wang X;Kang Y;Sun H;Liang Y;Liu J;Su Z;Dan J;Luo L;Yue T;Wang J;Zhang W
糖含量和细菌污染水平分别是食品健康和安全的重要指标。因此，构建一种既能检测葡萄糖又能检测细菌活性的方法是非常重要的。本文中，天然化合物（没食子酸和葡萄糖氧化酶）组装成纳米酶（GOx@GA-Fe（ii））用于温和级联检测。纳米酶催化葡萄糖产生过氧化氢，然后将其转化为·OH并从蓝色氧化TMB氧化无色TMB。在最佳条件下，葡萄糖浓度在1-500 μM范围内呈良好的线性关系（R2 = 0.993），最低检测浓度为0.43 μM。根据细菌代谢对葡萄糖的消耗，应用级联反应检测了5种常见细菌的活力。因此，设计了一种基于天然自组装纳米酶的级联方法来监测食品质量。
Sugar content and bacterial contamination levels are important indicators for the health and safety of food, respectively. Therefore, it is important to construct a method that can detect both glucose and bacterial activity. Herein, natural compounds (gallic acid and glucose oxidase) were assembled into nanozyme (GOx@GA-Fe (ii)) for mild cascade detection. The nanozyme catalyzes glucose to produce hydrogen peroxide, which is then converted to ·OH and oxidized colorless TMB from blue oxidized TMB. Under the optimal conditions, the method has a good linear relationship in the glucose concentration range of 1–500 μM (R2 = 0.993) with minimum detection concentration of 0.43 μM. Based on the glucose consumption of bacteria metabolism, the cascade reaction was then applied to detect the viability of 5 common bacteria. As such, a cascade method based on a natural self-assembled nanozyme was fabricated to monitor the quality of food.
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DOI：
10.3390/s17081866
发表时间：
2017-08-12
期刊：
Sensors (Basel, Switzerland)
影响因子：
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